Central European Institute of Technology (CEITEC), Masaryk University , 625 00 Brno, Czech Republic.
Anal Chem. 2014 Jan 21;86(2):982-6. doi: 10.1021/ac402823n. Epub 2014 Jan 6.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 × 100 pixel MS image in ~11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）成像技术是一种快速发展的技术，在生物科学领域具有巨大的潜力。在过去，获取单个高分辨率 MS 图像可能需要数天的时间。尽管最近引入了高重复率激光器来提高轴向 TOF MS 仪器的样品通量，但获得高分辨率图像仍然需要几个小时。本文表明，通过在激光束沿样品表面高速精确扫描的反射镜，可以大大提高基于 TOF MS 的组织成像的通量。配备扫描反射镜的实验室制造的轴向 MALDI-TOF MS 仪器利用 4 kHz UV 激光器，在 100 激光点/像素下，记录了 100×100 像素的 MS 图像，用时约 11 分钟。与配备 1 kHz 激光器的现代商业仪器相比，速度快了近一个数量级。
