Department of Oncology, Poznan University of Medical Sciences, ul. Szamarzewskiego 82/84, 60-569 Poznan, Poland.
Department of Biophysics, Poznan University of Medical Sciences, ul. Fredry 10, 61-701 Poznan, Poland.
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1548-53. doi: 10.1016/j.msec.2012.04.041. Epub 2012 Apr 28.
Allogeneic bone grafts are used on a large scale in surgeries. To avoid the risk of infectious diseases, allografts should be radiation-sterilized. So far, no international consensus has been achieved regarding the optimal radiation dose. Many authors suggest that bone sterilization deteriorates bone mechanical properties. However, no data on the influence of ionizing radiation on bone dynamic mechanical properties are available. Bovine femurs from 2-year old animal were machine cut and irradiated with the doses 10, 15, 25, 35, 45 and 50 kGy. Dynamic mechanical analysis was performed at 1-10 Hz at the temperature range of 0-350 °C in 3-point bending configuration. No statistically significant differences in storage modulus were observed. However, there were significant decreased values of loss modulus between the samples irradiated with doses of 10 (↓14.3%), 15, 45 and 50 kGy (↓33.2%) and controls. It was stated that increased irradiation dose decreases the temperature where collagen denaturation process starts and increases the temperature where the collagen denaturation process finishes. It was shown that activation energy of denaturation process is significantly higher for the samples irradiated with the dose of 50 kGy (615 kJ/mol) in comparison with control samples and irradiation with other doses (100-135 kJ/mol).
同种异体骨移植物在手术中被大规模使用。为了避免传染病的风险，同种异体移植物应该进行辐射消毒。到目前为止，对于最佳辐射剂量还没有达成国际共识。许多作者认为，骨消毒会恶化骨的机械性能。然而，目前还没有关于电离辐射对骨动态力学性能影响的数据。从 2 岁的动物身上取下牛股骨，用 10、15、25、35、45 和 50 kGy 的剂量进行机器切割和辐照。在 3 点弯曲配置下，在 0-350°C 的温度范围内以 1-10 Hz 的频率进行动态力学分析。存储模量没有观察到统计学上的显著差异。然而，在 10（↓14.3%）、15、45 和 50 kGy（↓33.2%）剂量辐照的样品和对照组之间，损耗模量值显著降低。有研究表明，增加辐照剂量会降低胶原蛋白变性过程开始的温度，并增加胶原蛋白变性过程结束的温度。结果表明，与对照组和其他剂量（100-135 kJ/mol）相比，50 kGy 剂量辐照的样品的变性过程的活化能显著更高（615 kJ/mol）。
