Ng R C, Roberts A N, Wilson R G, Latner A L, Turner G A
Br J Cancer. 1987 Mar;55(3):249-54. doi: 10.1038/bjc.1987.48.
The protein and glycoprotein composition of Triton X-100 extracts of breast biopsies from 17 women with benign breast disease and from 11 women with invasive breast carcinoma were investigated using electrophoresis in SDS-containing gradient polyacrylamide gels, followed by Coomassie Blue (CB) staining and the binding of radio-iodinated wheat germ agglutinin (WGA). Patterns were analysed after the CB-step for differences in protein composition, and after the WGA-step for differences in glycoprotein composition. Tissue extracts from patients with benign breast disease have less CB stained bands than similar extracts from the cancer patients. A particular consistent change was the appearance of an extra band at 58 Kdaltons in the cancer extracts. In contrast to the CB results, WGA detected less major bands, in the 40-60 Kd region, in the cancer extracts than at similar locations in benign extracts. Analysis of blood sera using the above techniques suggested that certain serum proteins could account for some of the WGA changes, but not the changes after CB staining. However, residual contamination of the specimens by blood proteins seemed unlikely because of the washing procedure used, unless these components were very strongly associated with the tissue. Differential synthesis of serum proteins by benign and malignant breast tissue may also explain some of our findings. Examination of the histopathology adjacent to the extracted tissue suggested that the degree of reduction in WGA-binding may be related to the extent of local invasiveness. Other animal and human studies suggest that reduced glycosylation of tumour-associated proteins may be linked to increased malignancy. The current findings may reflect a general pattern of change in tumour glycoprotein composition linked to malignant expression.
采用含十二烷基硫酸钠（SDS）的梯度聚丙烯酰胺凝胶电泳，继以考马斯亮蓝（CB）染色及放射性碘化麦胚凝集素（WGA）结合的方法，对17例患良性乳腺疾病的女性和11例浸润性乳腺癌女性的乳腺活检组织经曲拉通X - 100提取后的蛋白质和糖蛋白组成进行了研究。在CB染色步骤后分析蛋白质组成差异的图谱，在WGA结合步骤后分析糖蛋白组成差异的图谱。良性乳腺疾病患者的组织提取物经CB染色后的条带比癌症患者的类似提取物少。一个特别一致的变化是癌症提取物中在58千道尔顿处出现了一条额外的条带。与CB染色结果相反，WGA检测到癌症提取物中40 - 60千道尔顿区域的主要条带比良性提取物中相同位置的少。使用上述技术对血清进行分析表明，某些血清蛋白可以解释部分WGA变化，但不能解释CB染色后的变化。然而，由于所采用的洗涤程序，标本受血液蛋白残留污染的可能性似乎不大，除非这些成分与组织紧密结合。良性和恶性乳腺组织对血清蛋白的差异合成也可能解释我们的一些发现。对提取组织相邻部位的组织病理学检查表明，WGA结合减少的程度可能与局部侵袭程度有关。其他动物和人体研究表明，肿瘤相关蛋白糖基化减少可能与恶性程度增加有关。目前的发现可能反映了与恶性表达相关的肿瘤糖蛋白组成变化的一般模式。
