Sariban E, Mitchell T, Kufe D
Blood. 1987 May;69(5):1437-40.
The murine sarcoma virus 3611 contains the transforming v-raf gene that has partial nucleotide homology with the src family of tyrosine kinase-encoding oncogenes. Although this virus induces fibrosarcomas in mice, a recombinant murine retrovirus carrying both the raf and myc oncogenes induces immunoblastic lymphomas and immortalizes mouse macrophages in vitro. The present study has thus monitored the expression of c-raf in human hematopoietic cells. The results demonstrate the presence of a 3.6-kb c-raf transcript in HL-60 promyelocytic leukemic cells. The induction of HL-60 cell differentiation along the monocytic or granulocytic lineages had no detectable effect on the level of c-raf transcripts. Furthermore, in contrast to c-myc and c-fms expression, inhibition of protein synthesis with cycloheximide had no detectable effect on c-raf expression. Similar levels of c-rafRNA were also found in other human cell lines derived from myeloid, B cell, and T cell tumors, as well as in normal granulocytes, monocytes, and macrophages. These findings suggest that the c-raf protooncogene is widely expressed in multiple hematopoietic lineages.
鼠肉瘤病毒3611含有转化型v-raf基因，该基因与编码酪氨酸激酶的癌基因src家族具有部分核苷酸同源性。虽然这种病毒在小鼠中诱发纤维肉瘤，但携带raf和myc癌基因的重组鼠逆转录病毒可诱发免疫母细胞淋巴瘤，并使小鼠巨噬细胞在体外永生化。因此，本研究监测了人造血细胞中c-raf的表达。结果表明，HL-60早幼粒细胞白血病细胞中存在3.6kb的c-raf转录本。HL-60细胞沿单核细胞或粒细胞谱系的分化诱导对c-raf转录本水平没有可检测到的影响。此外，与c-myc和c-fms的表达相反，用放线菌酮抑制蛋白质合成对c-raf表达没有可检测到的影响。在源自髓系、B细胞和T细胞肿瘤的其他人类细胞系以及正常粒细胞、单核细胞和巨噬细胞中也发现了相似水平的c-rafRNA。这些发现表明，c-raf原癌基因在多个造血谱系中广泛表达。
