Kozlowska Justyna, Rivett Damian W, Vermeer Louic S, Carroll Mary P, Bruce Kenneth D, Mason A James, Rogers Geraint B
Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK.
Cystic Fibrosis Unit, Southampton University Hospitals NHS Trust, Southampton, SO16 6YD, UK.
Metabolomics. 2013 Dec;9(6). doi: 10.1007/s11306-013-0538-5.
Chronic polymicrobial lung infections in adult cystic fibrosis patients are typically dominated by high levels of . Determining the impact of growth on airway secretion composition is fundamental to understanding both the behaviour of this pathogen , and its relationship with other potential colonising species. We hypothesised that the marked differences in the phenotypes of clinical isolates would be reflected in the metabolite composition of spent culture media. H NMR spectroscopy was used to characterise the impact of growth on a synthetic medium as part of an CF lower airways model system. Comparisons of 15 CF clinical isolates were made and four distinct metabolomic clusters identified. Highly significant relationships between isolate cluster membership and both patient lung function (FEV) and spent culture pH were identified. This link between clinical isolate growth behaviour and FEV indicates characterisation of growth may find application in predicting patient lung function while the significant divergence in metabolite production and consumption observed between CF clinical isolates suggests dominant isolate characteristics have the potential to play both a selective role in microbiota composition and influence pseudomonal behaviour .
成年囊性纤维化患者的慢性多微生物肺部感染通常以高水平的……为主导。确定……生长对气道分泌物成分的影响对于理解这种病原体的行为及其与其他潜在定植物种的关系至关重要。我们假设临床分离株表型的显著差异将反映在废弃培养基的代谢物组成中。作为囊性纤维化下呼吸道模型系统的一部分，使用核磁共振氢谱来表征……生长对合成培养基的影响。对15株囊性纤维化临床分离株进行了比较，并确定了四个不同的代谢组学簇。确定了分离株簇成员与患者肺功能（第一秒用力呼气容积）和废弃培养基pH值之间的高度显著关系。临床分离株生长行为与第一秒用力呼气容积之间的这种联系表明，……生长的表征可能在预测患者肺功能方面有应用，而囊性纤维化临床分离株之间观察到的代谢物产生和消耗的显著差异表明，优势分离株特征有可能在微生物群组成中发挥选择性作用并影响铜绿假单胞菌的行为。
