Bepler G, Carney D N, Gazdar A F, Minna J D
Cancer Res. 1987 May 1;47(9):2371-5.
Production and secretion of neuroendocrine peptides by small cell lung cancer (SCLC) has been detected in the past years. Most recently the role of bombesin as an autocrine/paracrine growth modifier has been demonstrated. We used the soft agarose clonogenic assay to evaluate the influence of other neuroendocrine peptides on the in vitro proliferation of SCLC cell lines. Neuroendocrine peptides tested were adrenocorticotropic hormone, arginine vasopressin, calcitonin, glucagon, kassinin, neurotensin, physalaemin, somatostatin, and substance P. Experiments were carried out in serum-free and serum-supplemented media with and without serum-free incubation periods. Our results indicated that the amphibian undecapeptide physalaemin inhibits the clonal and mass culture growth of SCLC cell lines at picomolar concentrations. All other neuroendocrine peptides failed to influence SCLC growth in the test systems used. These results suggest a growth regulating effect of physalaemin and a potential new form of neuroendocrine peptide therapy for SCLC.
在过去几年中已检测到小细胞肺癌（SCLC）产生并分泌神经内分泌肽。最近，蛙皮素作为一种自分泌/旁分泌生长调节剂的作用已得到证实。我们使用软琼脂克隆形成试验来评估其他神经内分泌肽对SCLC细胞系体外增殖的影响。所测试的神经内分泌肽有促肾上腺皮质激素、精氨酸加压素、降钙素、胰高血糖素、蛙皮缩胆囊素、神经降压素、 Physalaemin、生长抑素和P物质。实验在无血清和补充血清的培养基中进行，有无无血清孵育期。我们的结果表明，两栖类十一肽Physalaemin在皮摩尔浓度下可抑制SCLC细胞系的克隆生长和大量培养生长。在所用的测试系统中，所有其他神经内分泌肽均未影响SCLC的生长。这些结果表明Physalaemin具有生长调节作用，并提示了一种潜在的SCLC神经内分泌肽治疗新形式。
