在国立卫生研究院临床中心建立骨髓基质细胞移植项目。
Establishing a bone marrow stromal cell transplant program at the National Institutes of Health Clinical Center.
机构信息
1 Department of Transfusion Medicine, Clinical Center, National Institutes of Health , Bethesda, Maryland.
出版信息
Tissue Eng Part B Rev. 2014 Jun;20(3):200-5. doi: 10.1089/ten.TEB.2013.0529. Epub 2014 Feb 7.
Abstract
A repository of cryopreserved bone marrow stromal cell (BMSC) products prepared from marrow aspirates of healthy subjects has been created and is being used to treat patients with inflammatory bowel disease, cardiovascular disease, and acute graft-versus-host disease following allogeneic hematopoietic stem cell transplantation. New methods of manufacturing BMSCs are being investigated including the use of an automated bioreactor for BMSC expansion and the replacement of fetal bovine serum with human platelet lysate as a media supplement. Efforts are also being made to identify markers that can be used to assess the potency of BMSCs.
摘要
已建立了一个冷冻保存的骨髓基质细胞（BMSC）产品库，这些产品是从健康供体的骨髓抽吸物中制备的，用于治疗炎症性肠病、心血管疾病和异基因造血干细胞移植后急性移植物抗宿主病的患者。目前正在研究新的 BMSC 制造方法，包括使用自动化生物反应器进行 BMSC 扩增，以及用人血小板裂解物替代胎牛血清作为培养基补充。此外，还正在努力确定可用于评估 BMSC 效力的标志物。
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