Dumont F J
Cell Immunol. 1987 Apr 1;105(2):355-65. doi: 10.1016/0008-8749(87)90083-9.
The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we compared the effect of the immunosuppressant cyclosporine A (CsA) on Ly-6A induction by IFN and concanavalin A (Con A). The augmentation of Ly-6A expression produced by treatment of T cells with IFN-alpha/beta or IFN-gamma was found not to be affected by CsA concentrations up to 2 micrograms/ml. In contrast, at doses as low as 50 ng/ml, CsA prevented the enhancement of Ly-6A expression in Con A-treated T-cell cultures. Culture supernatant transfer experiments were performed to further explore this effect of CsA. It was found that supernatants from Con A-activated T cells enhanced Ly-6A expression in resting T cells. This activity could be neutralized with an anti-IFN-gamma monoclonal antibody. Supernatants from T cells treated with Con A in presence of CsA lacked Ly-6A-enhancing activity. Taken together, these data suggest that the inhibition by CsA of Ly-6A induction in Con A-treated T cells reflects the known inhibitory effects of the drug on IFN-gamma secretion. This may imply the existence in T cells of an autocrine circuit involving IFN-gamma and regulating Ly-6A expression.
小鼠Ly-6A细胞表面抗原通常存在于一小部分成熟T细胞上。已证明该标志物在有丝分裂原激活的T细胞上高度表达。我们发现，通过与IFN-α/β或IFN-γ孵育，静止T细胞中Ly-6A的表达也会显著增加。在此，我们比较了免疫抑制剂环孢素A（CsA）对IFN和伴刀豆球蛋白A（Con A）诱导Ly-6A的影响。发现用IFN-α/β或IFN-γ处理T细胞所产生的Ly-6A表达增强不受高达2微克/毫升的CsA浓度影响。相反，低至50纳克/毫升的剂量时，CsA可阻止Con A处理的T细胞培养物中Ly-6A表达的增强。进行了培养上清液转移实验以进一步探究CsA的这种作用。发现Con A激活的T细胞的上清液可增强静止T细胞中Ly-6A的表达。这种活性可用抗IFN-γ单克隆抗体中和。在CsA存在下用Con A处理的T细胞的上清液缺乏Ly-6A增强活性。综上所述，这些数据表明CsA对Con A处理的T细胞中Ly-6A诱导的抑制反映了该药物对IFN-γ分泌的已知抑制作用。这可能意味着T细胞中存在一个涉及IFN-γ并调节Ly-6A表达的自分泌回路。
