Department of Orthopaedics, Anhui Provincial Hospital, No, 17, Road Lujiang, Hefei 230001, China.
Eur J Med Res. 2013 Dec 27;18(1):62. doi: 10.1186/2047-783X-18-62.
Intervertebral disc (IVD) cells experience a broad range of physicochemical stimuli under physiologic conditions, including alterations in their osmotic environment. At present, the molecular mechanisms underlying osmotic regulation in IVD cells are poorly understood. This study aims to screen genes affected by changes in osmotic pressure in cells of subjects aged 29 to 63 years old, with top-scoring pair (TSP) method.
Gene expression data set GSE1648 was downloaded from Gene Expression Omnibus database, including four hyper-osmotic stimuli samples, four iso-osmotic stimuli samples, and three hypo-osmotic stimuli samples. A novel, simple method, referred to as the TSP, was used in this study. Through this method, there was no need to perform data normalization and transformation before data analysis.
A total of five pairs of genes ((CYP2A6, FNTB), (PRPF8, TARDBP), (RPS5, OAZ1), (SLC25A3, NPM1) and (CBX3, SRSF9)) were selected based on the TSP method. We inferred that all these genes might play important roles in response to osmotic stimuli and age in IVD cells. Additionally, hyper-osmotic and iso-osmotic stimuli conditions were adverse factors for IVD cells.
We anticipate that our results will provide new thoughts and methods for the study of IVD disease.
椎间盘（IVD）细胞在生理条件下会经历广泛的物理化学刺激，包括渗透环境的改变。目前，IVD 细胞中渗透调节的分子机制还知之甚少。本研究旨在使用 TSP 方法筛选出 29 至 63 岁受试者的细胞中渗透压变化影响的基因。
从基因表达综合数据库中下载基因表达数据集 GSE1648，其中包含四个高渗刺激样本、四个等渗刺激样本和三个低渗刺激样本。本研究使用了一种新颖的简单方法 TSP，在数据分析之前无需对数据进行归一化和转换。
根据 TSP 方法，共选择了五对基因（CYP2A6，FNTB）、（PRPF8，TARDBP）、（RPS5，OAZ1）、（SLC25A3，NPM1）和（CBX3，SRSF9）。我们推断，所有这些基因可能在 IVD 细胞对渗透刺激和年龄的反应中发挥重要作用。此外，高渗和等渗刺激条件对 IVD 细胞是不利因素。
我们预计我们的结果将为 IVD 疾病的研究提供新的思路和方法。
