Li Qian-Yu, Wei Xu-Dong, Chen Lin, Yin Qing-Song
Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China.
Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Dec;21(6):1399-402. doi: 10.7534/j.issn.1009-2137.2013.06.007.
This study was purposed to investigate the effect of blocking Ras/Erk signaling pathway on expression of important transcription factor c-fos, c-jun and TAK1 gene in primary acute lymphocytic leukemia (ALL) cells. The best effective concentration and effect time of PD98059 were screened; the expression levels of c-fos, c-jun and TAK1 in primary cultured cells of normal persons, primary cultured ALL cells and primary cultured ALL cells treated by PD98059 were detected by SYBR GreenI real-time quantitative-PCR. The results showed that before treatment by PD98059 the expression levels of c-fos and TAK1 mRNA were significantly up-regulated in primary cultured ALL cells as compared with primary cultured cells of normal persons (P = 0.014 and P = 0.017 respectively). After treatment by PD98059, the expression levels of c-fos, c-jun mRNA decreased in all 7 serum samples, while expression of TAK1 was down-regulated in 5 samples, and up-regulated in 2 samples. After treatment with PD98059, there was no statistical difference of c-fos, c-jun and TAK1 expression levels in primary cultured ALL cells and primary cultured normal cells. It is concluded that the c-fos and TAK1 activity of primary cultured ALL cells increases, and blocking the Ras/Erk signaling pathway of ALL cells can lead to obvious decrease of important transcription factors c-fos, c-jun, TAK1 genes expression.
本研究旨在探讨阻断Ras/Erk信号通路对原代急性淋巴细胞白血病（ALL）细胞中重要转录因子c-fos、c-jun和TAK1基因表达的影响。筛选出PD98059的最佳有效浓度和作用时间；采用SYBR GreenI实时定量PCR检测正常人原代培养细胞、原代培养ALL细胞以及经PD98059处理的原代培养ALL细胞中c-fos、c-jun和TAK1的表达水平。结果显示，在PD98059处理前，原代培养ALL细胞中c-fos和TAK1 mRNA的表达水平与正常人原代培养细胞相比显著上调（分别为P = 0.014和P = 0.017）。经PD98059处理后，7份血清样本中c-fos、c-jun mRNA的表达水平均下降，而TAK1的表达在5份样本中下调，2份样本中上调。PD98059处理后，原代培养ALL细胞与原代培养正常细胞中c-fos、c-jun和TAK1的表达水平无统计学差异。结论：原代培养ALL细胞的c-fos和TAK1活性增强，阻断ALL细胞的Ras/Erk信号通路可导致重要转录因子c-fos、c-jun、TAK1基因表达明显下降。
