Ma Ling-Di, Lu Xu-Zhang, Zhu Zhi-Chao, Jiang Li-Jia, Zhou Min, Qian Si-Xuan, Li Jian-Yong
Lab Center, Changzhou No.2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu Province, China. E-mail:
Department of Hematology, Changzhou No.2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Dec;21(6):1429-34. doi: 10.7534/j.issn.1009-2137.2013.06.012.
This study was aimed to analyze the expression of NKG2D ligands in human leukemic cells and to investigate the effects of matrine on NKG2D ligand expression. The expressions of NKG2D ligand MICA/B, ULBP1-3 in several human leukemia cell lines (K562, OUN-1, U937 and K562/AO2), as well as primary leukemic cells isolated from malignant leukemia patients were analyzed by flow cytometry. After treatment with different doses of matrine, the expression level of NKG2D ligands in these leukemic cells was detected by FCM. The results indicated that NKG2D ligand expression was detected in both the leukemia cell lines and primary malignant leukemic cells. Generally, the expression of ULBP was high or obviously higher than that of MICA/B in leukemia cell lines and primary leukemic cells. The expression pattern of NKG2D ligands was different among these cells, possibly due to the different types of leukemia. Not all the expression of NKG2D ligands was upregulated after matrine treatment. Much higher expressions of ULBP2 and ULBP3 were found in K562 cells, compared to the other cell lines, which partly contributes to the higher sensitivity of K562 cells to NK cytotoxicity as target cells. It is concluded that there is universal expression of NKG2D ligand in leukemia cells. The high ULBP expression is prevalent in human leukemia cells. Matrine has the potential to induce the expression of NKG2D ligands in leukemia cells.
本研究旨在分析人白血病细胞中NKG2D配体的表达情况，并探讨苦参碱对NKG2D配体表达的影响。采用流式细胞术分析了几种人白血病细胞系（K562、OUN-1、U937和K562/AO2）以及从恶性白血病患者分离的原代白血病细胞中NKG2D配体MICA/B、ULBP1-3的表达。用不同剂量的苦参碱处理后，通过流式细胞术检测这些白血病细胞中NKG2D配体的表达水平。结果表明，在白血病细胞系和原代恶性白血病细胞中均检测到NKG2D配体表达。一般来说，在白血病细胞系和原代白血病细胞中，ULBP的表达较高或明显高于MICA/B。这些细胞中NKG2D配体的表达模式不同，可能是由于白血病类型不同。苦参碱处理后并非所有NKG2D配体的表达均上调。与其他细胞系相比，K562细胞中ULBP2和ULBP3的表达更高，这部分导致了K562细胞作为靶细胞对NK细胞毒性的更高敏感性。结论是白血病细胞中普遍存在NKG2D配体表达。ULBP高表达在人白血病细胞中普遍存在。苦参碱有诱导白血病细胞中NKG2D配体表达的潜力。
