Chen Tong-Qing, Chen Chang-Chun, Wang Jun-Xian, Chen Wen-Feng, Gu Xiao-Mei, Xu Rong-Sheng, Li Zhen-Xing, Wu Da-Xin, Zhang Wen-Sheng, Zhao Yan
Department of Blood Transfusion, The Second People Hospital of Anhui Province, Hefei 230011,Anhui Province, China. E-mail:
Department of Neurology, The Second People Hospital of Anhui Province, Hefei 230011,Anhui Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Dec;21(6):1612-6. doi: 10.7534/j.issn.1009-2137.2013.06.046.
The purpose of this study was to investigate the effect of glutathione (GSH) on blood coagulation. The normal plasma samples and mixed plasma samples were taken randomly, and into which the normal dose and different concentration of GSH were added. The prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and thrombin time (TT) were detected by using coagulation method before and after treatment with GSH. The detection results of normal plasma and mixed plasma containing GSH of different concentration were compared and analyzed with linear regression. The results showed that the APTT and FIB values of the plasma containing 2.5 mg/L glutathione or more, PT values of the plasma containing 10 mg/L glutathione or more, and TT values of the plasma containing 1250 mg/L glutathione or more were significantly different from those results of normal plasma or mixed plasma (P < 0.01) . There was a linear relation between all of the detection results of PT,APTT, FIB, TT and glutathione concentrations. The results of TT, APTT, PT and FIB detection in patient plasma were statistically different (P < 0.01) before and after treatment with normal concentration GSH. It is concluded that glutathione can influence detection results of coagulation function.
本研究旨在探讨谷胱甘肽（GSH）对血液凝固的影响。随机采集正常血浆样本和混合血浆样本，并向其中加入正常剂量及不同浓度的GSH。采用凝血法检测GSH处理前后的凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、纤维蛋白原（FIB）和凝血酶时间（TT）。对含不同浓度GSH的正常血浆和混合血浆的检测结果进行线性回归比较分析。结果显示，含2.5mg/L及以上谷胱甘肽的血浆的APTT和FIB值、含10mg/L及以上谷胱甘肽的血浆的PT值、含1250mg/L及以上谷胱甘肽的血浆的TT值与正常血浆或混合血浆的结果有显著差异（P<0.01）。PT、APTT、FIB、TT的所有检测结果与谷胱甘肽浓度之间存在线性关系。正常浓度GSH处理前后患者血浆的TT、APTT、PT和FIB检测结果有统计学差异（P<0.01）。结论是谷胱甘肽可影响凝血功能的检测结果。
