Division of Hog Cholera Research, Animal Health Research Institute, Council of Agriculture, No. 376, Chung-Cheng Rd., Tansui District, New Taipei City 251, Taiwan.
Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan; Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan.
Res Vet Sci. 2014 Feb;96(1):187-95. doi: 10.1016/j.rvsc.2013.11.020. Epub 2013 Dec 9.
Recently, it has been noted that porcine circovirus type 2 (PCV2) infection adversely affects the protective efficacy of Lapinized Philippines Coronel (LPC) vaccine, an attenuated strain of classical swine fever virus (CSFV), in pigs. In order to investigate the possible mechanisms of the PCV2-derived interference, an in vitro model was established to study the interaction of LPC virus (LPCV) and PCV2 in porcine alveolar macrophages (AMs). The results showed that PCV2 reduced the LPCV infection in AMs and the levels of PCV2-derived interference were dose-dependent. The PCV2-derived interference also reduced the replication level of LPCV in AMs. The full-length PCV2 DNA and its fragment DNA C9 CpG-ODN were involved in the reduction of LPCV infection in AMs, whereas UV-inactivated PCV2 was not. In addition, a moderate negative correlation between the LPCV antigen-containing rate and IFN-γ production was observed, and had a dose-dependent trend with the level of PCV2-inoculation. The results of the present study may partially explain how PCV2 infection interferes with the efficacy of LPC vaccine.
最近有研究指出，猪圆环病毒 2 型（PCV2）感染会降低拉皮尼菲律宾科罗内尔株（LPC）疫苗——一种猪瘟病毒（CSFV）弱毒疫苗的保护效力。为了探究 PCV2 干扰的可能机制，本研究建立了体外模型，以研究在猪肺泡巨噬细胞（AMs）中 LPC 病毒（LPCV）与 PCV2 的相互作用。结果表明，PCV2 降低了 AMs 中的 LPCV 感染，PCV2 干扰水平呈剂量依赖性。PCV2 干扰还降低了 LPCV 在 AMs 中的复制水平。全长 PCV2 DNA 及其片段 DNA C9 CpG-ODN 参与了 AMs 中 LPCV 感染的减少，而紫外线灭活的 PCV2 则没有。此外，还观察到 LPCV 抗原含量与 IFN-γ 产生之间存在中度负相关，且与 PCV2 接种水平呈剂量依赖性趋势。本研究结果可能部分解释了 PCV2 感染如何干扰 LPC 疫苗的效力。
