Inhibition by cytochalasin B of DNA synthesis in a thermosensitive anchorage-independent growth mutant.

After a shift from a nonpermissive to a permissive temperature, synchronized DNA synthesis and cell division were observed in a cold-sensitive anchorage-independent growth mutant (cs-17-25) of Chinese hamster lung cells in Methocel culture. Only 15 min exposure to the permissive temperature was sufficient for induction of DNA synthesis in the cells. A low dose of actinomycin D (0.02 micrograms/ml) or cytochalasin B (5 micrograms/ml) was able to inhibit the DNA synthesis when added at an early period after the temperature shift. The inhibitory effects of actinomycin D and cytochalasin B on RNA and protein synthesis were very similar at both temperatures. The degree of multinucleation caused by cytochalasin B was altered quickly depending on the incubation temperature. These results suggest that stimulation of this mutant involves an obligatory actin-mediated step closely correlated to transcription of early mRNAs.