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在人类糖尿病皮肤组织中鉴定出5型17β-羟基类固醇脱氢酶（AKR1C3）蛋白表达降低。

Decreased Expression of Type 5 17β-Hydroxysteroid Dehydrogenase (AKR1C3) Protein Identified in Human Diabetic Skin Tissue.

作者信息

Cho Moon-Kyun

机构信息

Department of Dermatology, Soonchunhyang University College of Medicine, Seoul, Korea.

出版信息

Ann Dermatol. 2013 Nov;25(4):423-7. doi: 10.5021/ad.2013.25.4.423. Epub 2013 Nov 30.

DOI:10.5021/ad.2013.25.4.423
PMID:24371388
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3870209/
Abstract

BACKGROUND

Diabetes is characterized by chronic hyperglycemia, and hyperglycemia can increase reactive oxygen species (ROS) production from the mitochondrial electron transport chain. The formation of ROS in cells induces oxidative stress and activates oxidative damage-inducing genes. There is no research on the protein levels of oxidative damage-related genes AKR1C3 in human diabetic skin. We explored the expression of AKR1C3 in diabetic skin compared with normal skin tissue.

OBJECTIVE

To compare the expression of AKR1C3 in normal skin versus diabetic skin.

METHODS

AKR1C3 expression was evaluated by western blotting in 6 diabetic skin tissue samples and 6 normal skin samples. Immunohistochemical staining was carried out to analyze AKR1C3 expression in the 6 diabetic skin tissue samples (July 2009 to December 2011; Department of Plastic and Reconstructive Surgery at Soonchunhyang University Seoul Hospital, Seoul, Korea).

RESULTS

The western blotting showed a significant reduction in AKR1C3 protein expression in diabetic skin tissue compared to normal tissue. Immunohistochemical examination of AKR1C3 showed that it was weakly expressed in all diabetic skin samples.

CONCLUSION

We believe that AKR1C3 is related to diabetic skin in altered metabolic states which elevate ROS production.

摘要

背景

糖尿病的特征为慢性高血糖，且高血糖会增加线粒体电子传递链中活性氧（ROS）的生成。细胞内ROS的形成会诱导氧化应激并激活氧化损伤诱导基因。目前尚无关于人类糖尿病皮肤中氧化损伤相关基因AKR1C3蛋白水平的研究。我们探讨了与正常皮肤组织相比，AKR1C3在糖尿病皮肤中的表达情况。

目的

比较正常皮肤与糖尿病皮肤中AKR1C3的表达。

方法

采用蛋白质印迹法评估6例糖尿病皮肤组织样本和6例正常皮肤样本中AKR1C3的表达。对6例糖尿病皮肤组织样本（2009年7月至2011年12月；韩国首尔顺天乡大学首尔医院整形与重建外科）进行免疫组织化学染色，以分析AKR1C3的表达。

结果

蛋白质印迹法显示，与正常组织相比，糖尿病皮肤组织中AKR1C3蛋白表达显著降低。AKR1C3的免疫组织化学检查表明，其在所有糖尿病皮肤样本中均呈弱表达。

结论

我们认为，AKR1C3与代谢状态改变且ROS生成增加的糖尿病皮肤有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b3e/3870209/cd23db9d0e0a/ad-25-423-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b3e/3870209/265729fe942f/ad-25-423-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b3e/3870209/4232337d0725/ad-25-423-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b3e/3870209/cd23db9d0e0a/ad-25-423-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b3e/3870209/265729fe942f/ad-25-423-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b3e/3870209/4232337d0725/ad-25-423-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b3e/3870209/cd23db9d0e0a/ad-25-423-g003.jpg

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