Kim Chul Han
Department of Plastic and Reconstructive Surgery, Soonchunhyang University College of Medicine, Seoul, Korea.
Arch Plast Surg. 2013 Sep;40(5):517-21. doi: 10.5999/aps.2013.40.5.517. Epub 2013 Sep 13.
Diabetes is characterized by chronic hyperglycemia, which can increase reactive oxygen species (ROS) production by the mitochondrial electron transport chain. The formation of ROS induces oxidative stress and activates oxidative damage-inducing genes in cells. No research has been published on oxidative damage-related extracellular superoxide dismutase (EC-SOD) protein levels in human diabetic skin. We investigated the expression of EC-SOD in diabetic skin compared with normal skin tissue in vivo.
The expression of EC-SOD protein was evaluated by western blotting in 6 diabetic skin tissue samples and 6 normal skin samples. Immunohistochemical staining was also carried out to confirm the EC-SOD expression level in the 6 diabetic skin tissue samples.
The western blotting showed significantly lower EC-SOD protein expression in the diabetic skin tissue than in the normal tissue. Immunohistochemical examination of EC-SOD protein expression supported the western blotting analysis.
Diabetic skin tissues express a relatively small amount of EC-SOD protein and may not be protected against oxidative stress. We believe that EC-SOD is related to the altered metabolic state in diabetic skin, which elevates ROS production.
糖尿病的特征是慢性高血糖，这会增加线粒体电子传递链产生的活性氧（ROS）。ROS的形成会诱导氧化应激并激活细胞中的氧化损伤诱导基因。关于人类糖尿病皮肤中氧化损伤相关的细胞外超氧化物歧化酶（EC-SOD）蛋白水平，尚无相关研究发表。我们在体内研究了与正常皮肤组织相比，糖尿病皮肤中EC-SOD的表达情况。
通过蛋白质印迹法评估6份糖尿病皮肤组织样本和6份正常皮肤样本中EC-SOD蛋白的表达。还进行了免疫组织化学染色，以确认6份糖尿病皮肤组织样本中EC-SOD的表达水平。
蛋白质印迹法显示，糖尿病皮肤组织中EC-SOD蛋白表达明显低于正常组织。EC-SOD蛋白表达的免疫组织化学检查支持了蛋白质印迹分析结果。
糖尿病皮肤组织表达相对少量的EC-SOD蛋白，可能无法抵御氧化应激。我们认为，EC-SOD与糖尿病皮肤中代谢状态的改变有关，这种改变会增加ROS的产生。
