Lönn J, Starkhammar Johansson C, Kälvegren H, Brudin L, Skoglund C, Garvin P, Särndahl E, Ravald N, Richter A, Bengtsson T, Nayeri F
Division of Clinical Medicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden ; PEAS Institute, Linköping, Sweden.
Division of Cardiovascular Medicine, Department of Medical and Health Sciences, Linköping University, Centre for Oral Rehabilitation, Public Dental Health Care, County Council of Östergötland, Linköping, Sweden.
Results Immunol. 2011 Dec 30;2:7-12. doi: 10.1016/j.rinim.2011.12.002. eCollection 2011.
Hepatocyte growth factor (HGF) is an angiogenic, cardioprotective factor important for tissue and vascular repair. High levels of HGF are associated with chronic inflammatory diseases, such as coronary artery disease (CAD) and periodontitis, and are suggested as a marker of the ongoing atherosclerotic event in patients with CAD. Periodontal disease is more prevalent among patients with CAD than among healthy people. Recent studies indicate a reduced biological activity of HGF in different chronic inflammatory conditions. Biologically active HGF has high affinity to heparan sulfate proteoglycan (HSPG) on cell-membrane and extracellular matrix. The aim of the study was to investigate the serum concentration and the biological activity of HGF with ELISA and surface plasmon resonance (SPR), respectively, before and at various time points after percutaneous coronary intervention (PCI) in patients with CAD, and to examine the relationship with periodontal condition. The periodontal status of the CAD patients was examined, and the presence of P. gingivalis in periodontal pockets was analyzed with PCR. The HGF concentration was significantly higher, at all time-points, in patients with CAD compared to the age-matched controls (P< 0.001), but was independent of periodontal status. The HGF concentration and the affinity to HSPG adversely fluctuated over time, and the biological activity increased one month after intervention in patients without periodontitis. We conclude that elevated concentration of HGF but with reduced biological activity might indicate a chronic inflammatory profile in patients with CAD and periodontitis.
肝细胞生长因子（HGF）是一种血管生成性、心脏保护因子，对组织和血管修复很重要。高水平的HGF与慢性炎症性疾病有关，如冠状动脉疾病（CAD）和牙周炎，并且被认为是CAD患者正在发生的动脉粥样硬化事件的标志物。牙周病在CAD患者中比在健康人群中更普遍。最近的研究表明，在不同的慢性炎症状态下，HGF的生物活性降低。具有生物活性的HGF与细胞膜和细胞外基质上的硫酸乙酰肝素蛋白聚糖（HSPG）具有高亲和力。本研究的目的是分别采用酶联免疫吸附测定（ELISA）和表面等离子体共振（SPR）技术，调查CAD患者经皮冠状动脉介入治疗（PCI）前及术后不同时间点血清中HGF的浓度和生物活性，并研究其与牙周状况的关系。检查了CAD患者的牙周状况，并用聚合酶链反应（PCR）分析了牙周袋中牙龈卟啉单胞菌的存在情况。与年龄匹配的对照组相比，CAD患者在所有时间点的HGF浓度均显著更高（P<0.001），但与牙周状况无关。HGF浓度和对HSPG的亲和力随时间呈不利波动，在无牙周炎的患者中，干预后1个月生物活性增加。我们得出结论，HGF浓度升高但生物活性降低可能表明CAD和牙周炎患者存在慢性炎症状态。
