Siah A, McKenna P, Danger J M, Johnson G, Berthe F C J
British Columbia Centre for Aquatic Health Sciences, 871A Island Highway, Campbell River, BC, Canada V9W 2C2 ; Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.
Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 220 University Avenue, Charlottetown, PEI, Canada C1A 4P3.
Results Immunol. 2012 Apr 7;2:83-7. doi: 10.1016/j.rinim.2012.03.003. eCollection 2012.
The molecular mechanisms by which disseminated neoplasia (DN) is developed in soft shell clams Mya arenaria remain largely unknown. This study aims at quantifying Rho-like GTPase, RAS-Rho, RAS-related C3 botulinum (RAS C3), c-jun as well as c-myc transcript levels in clams sampled at North River (Charlottetown, Prince Edward Island, Canada). The transcripts were quantified using multiplex gene analysis (Quantigene(®) 2 Plex, Affymetrix) in 3 groups of clams: (1) Group C (healthy clams considered as control) with a low percentage of tetraploid hemocytes (<10%); (2) Group D (disease in development): individuals presenting a percentage of tetraploid cells ranging between 10% and 50%; (3) Group E (established disease): clams with a high percentage of tetraploid hemocytes (>50%). Data showed a down-regulation of Rho-like GTPase, Rho-like subfamily, RAS C3, c-jun and an up-regulation of c-myc gene expression. It is believed that a deregulation of the expression of these genes could partly unravel the molecular mechanisms involved in the development of DN in soft shell clams Mya arenaria. Further investigations should be pursued to determine the role of these gene products in clams' hemocytes.
在软壳蛤中发生播散性肿瘤（DN）的分子机制在很大程度上仍然未知。本研究旨在对从加拿大爱德华王子岛夏洛特敦北河采集的蛤类中Rho样GTP酶、RAS-Rho、RAS相关C3肉毒杆菌（RAS C3）、c-jun以及c-myc转录水平进行定量分析。使用多重基因分析（Quantigene® 2 Plex，Affymetrix）对3组蛤类的转录本进行定量分析：（1）C组（健康蛤类作为对照），四倍体血细胞比例低（<10%）；（2）D组（疾病发展期）：四倍体细胞比例在10%至50%之间的个体；（3）E组（确诊疾病组）：四倍体血细胞比例高（>50%）的蛤类。数据显示Rho样GTP酶、Rho样亚家族、RAS C3、c-jun表达下调，c-myc基因表达上调。据信这些基因表达的失调可能部分揭示了软壳蛤中DN发生所涉及的分子机制。应进一步开展研究以确定这些基因产物在蛤类血细胞中的作用。
