Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba, Japan.
Exp Dermatol. 2014 Feb;23(2):138-40. doi: 10.1111/exd.12313.
Recent in vivo studies have demonstrated involvement of the histamine H4 receptor in pruritus and skin inflammation. We previously reported that an H4 receptor antagonist attenuated scratching behaviour and improved skin lesions in an experimental model of atopic dermatitis. We also reported the expression of the H4 receptor in human epidermal tissues. In this study, we investigated the expression of H4 receptor mRNA and the function of the receptor in a culture system that mimics in vivo inflammation on the HaCaT human keratinocyte cell line. Increased expression of the H4 receptor was observed in HaCaT cells following differentiation. Treatment of HaCaT cells with histamine and TNFα enhanced the mRNA expression of interleukin (IL)-8. These increases in expression were significantly inhibited by the H4 receptor antagonist JNJ7777120. Our results indicate that IL-8 mRNA expression might be enhanced by histamine and TNFα via H4 receptor stimulation in keratinocytes.
最近的体内研究表明，组胺 H4 受体参与瘙痒和皮肤炎症。我们之前的研究报告表明，H4 受体拮抗剂可减轻特应性皮炎实验模型中的搔抓行为并改善皮肤损伤。我们还报告了 H4 受体在人表皮组织中的表达。在这项研究中，我们在模拟体内炎症的 HaCaT 人角质形成细胞系培养系统中研究了 H4 受体 mRNA 的表达及其功能。H4 受体的表达在 HaCaT 细胞分化后增加。组胺和 TNFα 处理 HaCaT 细胞可增强白细胞介素 (IL)-8 的 mRNA 表达。H4 受体拮抗剂 JNJ7777120 显著抑制这些表达的增加。我们的结果表明，组胺和 TNFα 可能通过角质形成细胞中的 H4 受体刺激增强 IL-8 mRNA 的表达。
