Department of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong; Center for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong; Department of Electronic Information Engineering, Tianjin University, Tianjin 300072, China.
Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong; Center for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong.
Biosens Bioelectron. 2014 May 15;55:141-8. doi: 10.1016/j.bios.2013.11.075. Epub 2013 Dec 10.
A localized surface plasmon resonance (LSPR) effect was used to distinguish cell concentration on ordered arrays of Au nanoparticles (NPs) on glass substrates. Human-derived retinal pigment epithelial RPE-1 cells with flatter bodies and higher confluency were compared with breast cancer MCF-7 cells. Nanosphere lithography was used to form Au NPs with average diameters of 500 and 60 nm in order to compare cell detection range, resonance peak shift, and cell concentration sensitivity. A larger cell concentration range was detected on the larger 500 nm Au NPs compared to 60 nm Au NPs (8.56 × 10(3)-1.09 × 10(6) vs. 3.43 × 10(4)-2.73 × 10(5)cells/ml). Resonance peak shift could distinguish RPE-1 from MCF-7 cells on both Au NPs. RPE-1 cells consistently displayed larger resonance peak shifts compared to MCF-7 cells until the detection became saturated at higher concentration. For both types of cells, higher concentration sensitivity in the range of ~10(4)-10(6)cells/ml was observed on 500 nm compared to 60 nm Au NPs. Our results show that cells on Au NPs can be detected in a large range and at low concentration. Optimal cell sensing can be achieved by altering the dimensions of Au NPs according to different cell characteristics and concentrations.
利用局域表面等离子体共振（LSPR）效应来区分玻璃基底上有序排列的金纳米粒子（Au NPs）上的细胞浓度。与乳腺癌 MCF-7 细胞相比，我们比较了具有更扁平形状和更高融合度的人源性视网膜色素上皮 RPE-1 细胞。采用纳米球光刻法形成平均直径为 500nm 和 60nm 的 Au NPs，以比较细胞检测范围、共振峰位移和细胞浓度灵敏度。与 60nm Au NPs 相比，较大的 500nm Au NPs 上检测到的细胞浓度范围更大（8.56×10(3)-1.09×10(6) vs. 3.43×10(4)-2.73×10(5)cells/ml）。共振峰位移可以区分 Au NPs 上的 RPE-1 和 MCF-7 细胞。与 MCF-7 细胞相比，RPE-1 细胞的共振峰位移始终更大，直到在更高浓度下检测达到饱和。对于这两种类型的细胞，在 500nm Au NPs 上观察到的 ~10(4)-10(6)cells/ml 范围内的浓度灵敏度更高，而在 60nm Au NPs 上的灵敏度较低。我们的结果表明，可以在较大的浓度范围内和较低的浓度下检测 Au NPs 上的细胞。通过根据不同的细胞特性和浓度改变 Au NPs 的尺寸，可以实现最佳的细胞感应。
