1,25(OH)2D3 可抑制缺氧刺激时胎盘滋养层细胞中环氧化酶-2 的上调和血栓素的产生。
1,25(OH)2D3 suppresses COX-2 up-regulation and thromboxane production in placental trophoblast cells in response to hypoxic stimulation.
机构信息
Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA; Dept. of Obstetrics and Gynecology, First Hospital, Harbin Medical University, Harbin, China.
Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA.
出版信息
Placenta. 2014 Feb;35(2):143-5. doi: 10.1016/j.placenta.2013.12.002. Epub 2013 Dec 18.
Abstract
In this study, we determined if vitamin D could inhibit oxidative stress-induced thromboxane production by placental trophoblasts. Trophoblast isolated from normal placentas were stimulated with CoCl2, a hypoxic mimicking agent, with or without pretreatment of 1,25(OH)2D3. Soluble phospholipase-A2, metabolites of thromboxane-A2 and prostacyclin, and 8-isoprostane were measured. Expression of cyclooxygenase-1 (COX-1), COX-2, and heme oxygenase-1 (HO-1) were determined. We found that pretreatment of trophoblasts with 1,25(OH)2D3 significantly reduced 8-isoprostane and the ratio of thromboxane-A2 to prostacyclin production, and blocked COX-2 expression induced by CoCl2. These results provide evidence of the beneficial effects of vitamin D on placental trophoblasts.
摘要
在这项研究中，我们确定维生素 D 是否可以抑制胎盘滋养层细胞中氧化应激诱导的血栓素产生。用 CoCl2（缺氧模拟剂）刺激从正常胎盘分离的滋养层细胞，同时或不预先用 1,25(OH)2D3 处理。测量可溶性磷脂酶 A2、血栓素 A2 和前列环素的代谢物以及 8-异前列腺素。测定环加氧酶-1 (COX-1)、COX-2 和血红素加氧酶-1 (HO-1) 的表达。我们发现，用 1,25(OH)2D3 预处理滋养层细胞可显著降低 8-异前列腺素和血栓素 A2 与前列环素生成的比值，并阻断 CoCl2 诱导的 COX-2 表达。这些结果为维生素 D 对胎盘滋养层细胞的有益作用提供了证据。
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