State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China; College of Ocean and Earth Science, Xiamen University, Xiamen 361005, PR China.
College of Ocean and Earth Science, Xiamen University, Xiamen 361005, PR China.
Gene. 2014 Mar 10;537(2):294-301. doi: 10.1016/j.gene.2013.12.034. Epub 2013 Dec 27.
During a large-scale screen of the larval transcriptome library of the Portuguese oyster, Crassostrea angulata, the oyster gene RACK, which encodes a receptor of activated protein kinase C protein was isolated and characterized. The cDNA is 1,148 bp long and has a predicted open reading frame encoding 317 aa. The predicted protein shows high sequence identity to many RACK proteins of different organisms including molluscs, fish, amphibians and mammals, suggesting that it is conserved during evolution. The structural analysis of the Ca-RACK1 genomic sequence implies that the Ca-RACK1 gene has seven exons and six introns, extending approximately 6.5 kb in length. It is expressed ubiquitously in many oyster tissues as detected by RT-PCR analysis. The Ca-RACK1 mRNA expression pattern was markedly increased at larval metamorphosis; and was further increased along with Ca-RACK1 protein synthesis during epinephrine-induced metamorphosis. These results indicate that the Ca-RACK1 plays an important role in tissue differentiation and/or in cell growth during larval metamorphosis in the oyster, C. angulata.
在对葡萄牙牡蛎（Crassostrea angulata）幼虫转录组文库进行大规模筛选的过程中，分离并鉴定了牡蛎基因 RACK，该基因编码蛋白激酶 C 激活受体。cDNA 长 1148bp，具有一个预测的开放阅读框，编码 317 个氨基酸。预测的蛋白与许多不同生物体的 RACK 蛋白具有高度的序列同一性，包括软体动物、鱼类、两栖动物和哺乳动物，表明其在进化过程中是保守的。Ca-RACK1 基因的结构分析表明，该基因有七个外显子和六个内含子，长度约为 6.5kb。通过 RT-PCR 分析检测到，它在许多牡蛎组织中广泛表达。在幼虫变态期间，Ca-RACK1 mRNA 的表达模式明显增加；并且在肾上腺素诱导的变态过程中，随着 Ca-RACK1 蛋白的合成进一步增加。这些结果表明，Ca-RACK1 在牡蛎幼虫变态过程中的组织分化和/或细胞生长中发挥重要作用。
Zotero 插件