Cai Yuefeng, Hu Jie, Guo Yepeng, Shen Xin
Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China.
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China.
Biology (Basel). 2024 Mar 8;13(3):174. doi: 10.3390/biology13030174.
The receptor for activated protein kinase C1 (RACK1) belongs to the typical WD repeat family, which is extremely conservative and important in multiple signal transduction pathways related to growth and development that coordinate the intracellular role of various life activities. As a novel protein with versatile functions, it was found in a variety of organisms. In a previous study, we identified the RACK1 sequence of white shrimp from transcriptome data. In this study, we employed specialized bioinformatics software to conduct an in-depth analysis of RACK1 and compare its amino acid sequence homology with other crustaceans. Furthermore, we investigated the expression patterns of RACK1 at different developmental stages and tissues, as well as at various time points after exposure to Aroclor 1245, aiming to elucidate its function and potential response towards Aroclor 1245 exposure. The length of RACK1 is 957 nucleotides, which encodes 318 amino acids. Moreover, there were seven typical WD repeats in RACK1, which have more than a 96% sequence identity with the RACK1 proteins of . The results of tissue expression and spatiotemporal expression showed that it was significantly increased in the II and IV stages, but had a significant tissue specificity in the hepatopancreas, spermary, and muscle tissues of , adult stage. Compared to the control, RACK1 was significantly induced in zoea larvae exposed to Aroclor 1254 for 6, 10, 20, and 30 d ( < 0.05). These results suggested that RACK1 may play an important role in the larval development and environmental defense of .
活化蛋白激酶C1受体（RACK1）属于典型的WD重复蛋白家族，该家族在与生长和发育相关的多种信号转导途径中极为保守且重要，协调着细胞内各种生命活动的作用。作为一种具有多种功能的新型蛋白质，它在多种生物体中均有发现。在先前的一项研究中，我们从转录组数据中鉴定出了凡纳滨对虾的RACK1序列。在本研究中，我们使用专门的生物信息学软件对RACK1进行了深入分析，并将其氨基酸序列同源性与其他甲壳类动物进行了比较。此外，我们研究了RACK1在不同发育阶段和组织中的表达模式，以及暴露于多氯联苯混合物1245后不同时间点的表达情况，旨在阐明其功能以及对多氯联苯混合物1245暴露的潜在反应。RACK1的长度为957个核苷酸，编码318个氨基酸。此外，RACK1中有七个典型的WD重复序列，与凡纳滨对虾的RACK1蛋白具有超过96%的序列同一性。组织表达和时空表达结果表明，它在Ⅱ期和Ⅳ期显著增加，但在凡纳滨对虾成体阶段的肝胰腺、精巢和肌肉组织中具有显著的组织特异性。与对照组相比，暴露于多氯联苯混合物1254 6、10、20和30天的凡纳滨对虾溞状幼体中RACK1被显著诱导（P<0.05）。这些结果表明，RACK1可能在凡纳滨对虾的幼体发育和环境防御中发挥重要作用。
