Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Toxicol Lett. 2014 Mar 3;225(2):230-9. doi: 10.1016/j.toxlet.2013.12.017. Epub 2013 Dec 27.
This study aims to determine whether quercetin elicits a protective effect against the toxicity of chronic exposure to low-level DDVP using metabonomic technology. Rats were randomly assigned into the control, DDVP-treated, quercetin-treated, and quercetin plus DDVP-treated groups. DDVP and quercetin were given to rats daily via drinking water and gavage respectively for 90 days. Eighteen metabolites, including the biomarkers of DDVP exposure (dimethyl phosphate, DMP) and quercetin exposure (quercetin and isorhamnetina), were identified from the metabonomic profiles of rat urine using ultra-performance liquid chromatography-mass spectrometry. Compared with the control group, the DDVP-treated group showed statistically significantly increased intensities of indoxyl sulfate, estrone sulfate, cholic acid, deoxycholic acid, p-cresol, p-cresol sulfate, and orotic acid but decreased intensities of suberic acid, citric acid, sebacic acid, hippuric acid, taurine, phosphocreatine, 3-hydroxyanthranilic acid, and kynurenic acid. The tendency of the aforesaid metabolites to change was significantly ameliorated in the quercetin (50mg/kg·bw) plus DDVP (7.2mg/kg·bw)-treated group compared with the DDVP-treated group. However, the levels of these metabolites in the quercetin plus DDVP-treated groups were still significantly different from those of the control group. These results indicate that quercetin has a partial protective effect on DDVP-induced toxicity.
本研究旨在利用代谢组学技术确定槲皮素是否对慢性低水平敌敌畏暴露的毒性产生保护作用。大鼠被随机分为对照组、敌敌畏处理组、槲皮素处理组和槲皮素加敌敌畏处理组。敌敌畏和槲皮素分别通过饮水和灌胃每天给予大鼠，持续 90 天。使用超高效液相色谱-质谱法从大鼠尿液的代谢组学图谱中鉴定出 18 种代谢物，包括敌敌畏暴露的生物标志物（磷酸二甲酯、DMP）和槲皮素暴露的生物标志物（槲皮素和异鼠李素）。与对照组相比，敌敌畏处理组的吲哚硫酸、雌酮硫酸、胆酸、脱氧胆酸、对甲酚、对甲酚硫酸盐和尿嘧啶核苷的强度显著增加，但琥珀酸、柠檬酸、癸二酸、马尿酸、牛磺酸、磷酸肌酸、3-羟基邻氨基苯甲酸和犬尿氨酸的强度显著降低。与敌敌畏处理组相比，槲皮素（50mg/kg·bw）加敌敌畏（7.2mg/kg·bw）处理组上述代谢物的变化趋势明显改善。然而，槲皮素加敌敌畏处理组的这些代谢物水平仍与对照组有显著差异。这些结果表明槲皮素有部分保护作用，可以对抗敌敌畏引起的毒性。
