Key Laboratory of Ecology and Environment Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou, 510631, People's Republic of China.
Bull Environ Contam Toxicol. 2014 May;92(5):525-8. doi: 10.1007/s00128-013-1165-6. Epub 2013 Dec 28.
This study investigated the effect of ambient Cadmium (Cd) on haemocyte apoptosis of the shrimp, Penaeus monodon. Cellular response was determined in Cd-exposed (0, 0.05, 0.5 and 5 mg L(-1)) shrimp. Results showed that 0.05 mg L(-1) Cd(2+) had no significant effect on the haemocyte parameters during the 48 h exposure. Cadmium at doses of 0.5 and 5 mg L(-1) depressed the total haemocyte count (THC), and increased reactive oxygen species (ROS) production and apoptosis ratio in haemocytes. Esterase activity increased in shrimp exposed to 0.5 mg L(-1) Cd(2+) for 6 h, and decreased to the initial level later. Depressed esterase activity could be observed in shrimp after 24 and 48 h exposure to 5 mg L(-1) Cd(2+). These results demonstrated that Cd(2+) modified esterase activity and induced ROS generation, which led to haemocyte apoptosis and THC reduction. Oxidative stress is one of the induction mechanisms for Cd-caused apoptosis of shrimp haemocytes.
本研究调查了环境镉（Cd）对虾血细胞凋亡的影响。在暴露于 Cd（0、0.05、0.5 和 5 mg/L）的虾中确定了细胞反应。结果表明，在 48 小时暴露期间，0.05 mg/L Cd(2+) 对血细胞参数没有显著影响。0.5 和 5 mg/L Cd(2+) 抑制了总血细胞计数（THC），并增加了血细胞中活性氧物质（ROS）的产生和凋亡比例。在虾暴露于 0.5 mg/L Cd(2+) 6 小时后，酯酶活性增加，随后降低至初始水平。在虾暴露于 5 mg/L Cd(2+) 24 和 48 小时后，观察到酯酶活性降低。这些结果表明，Cd(2+) 改变了酯酶活性并诱导了 ROS 的产生，从而导致血细胞凋亡和 THC 减少。氧化应激是 Cd 诱导虾血细胞凋亡的诱导机制之一。
