基于光谱和分子建模研究 4-硫代-5-甲基尿苷与人血清白蛋白的相互作用。
Study on the interaction between 4-thio-5-methyluridine and human serum albumin by spectroscopy and molecular modeling.
机构信息
College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China.
College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:724-31. doi: 10.1016/j.saa.2013.11.113. Epub 2013 Dec 9.
The interaction between 4-thio-5-methyluridine and human serum albumin (HSA) under simulative physiological conditions has been studied by the methods of fluorescence, UV-VIS absorbance and circular dichroism (CD) spectroscopy. The results show that 4-thio-5-methyluridine has a static fluorescence quenching on human serum albumin (HSA). The main forces of both interactions have typical static interaction from thermodynamic data to the determination. The experimental result was in correspondence with molecular modeling theory.
在模拟生理条件下，通过荧光、紫外-可见吸收和圆二色性（CD）光谱法研究了 4-硫代-5-甲基尿苷与人血清白蛋白（HSA）之间的相互作用。结果表明，4-硫代-5-甲基尿苷对人血清白蛋白（HSA）具有静态荧光猝灭作用。从热力学数据到测定，两种相互作用的主要力都具有典型的静态相互作用。实验结果与分子建模理论相符。
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