Haholu A, Berber U, Karagöz B, Tuncel T, Bilgi O, Demirel D
Assoc. Prof. Aptullah Haholu MD, Pathology Department, Gulhane Military Medical Academy (GMMA), Haydarpasa Training Hospital, 34668 Uskudar/Istanbul, Turkey, tel. +905326718212, e-mail:
Pol J Pathol. 2013 Dec;64(4):247-52. doi: 10.5114/pjp.2013.39332.
The glutathione S-transferases (GST) are enzymes catalyzing reactions including carcinogens. GSTT1 and GSTM1 genes are polymorphic in humans. The relations between polymorphism of some GST genes and cancer have been reported. In this study, we aimed to investigate the distribution of GSTT1 and GSTM1 polymorphisms in a group of gastric cancer patients. The study group consisted of 50 patients (21 females, 29 males) with gastric adenocarcinoma from the archives of the pathology department of a training hospital. Fifty-seven healthy control subjects were included in the study as a control group. DNA was extracted from peripheral venous blood of control subjects and from the paraffin blocks of cases. Genotyping of GSTT1 and GSTM1 genes was performed with duplex polymerase chain reaction. No differences in the frequencies of GSTM1 or GSTT1 null genotypes were observed between patients and healthy subjects (GSTM1: 52% vs. 43.85%, OR = 1.27, 95% CI: 0.55-2.96; GSTT1: 38.46% vs. 28.07%, OR = 0.72, 95% CI: 0.25-1.96). Moreover, simultaneous carriage of both genotypes was almost identical in both groups. GSTM1 or GSTT1 null genotypes were not different in diffuse or intestinal type gastric cancer. Our data suggest that the GSTM1 and GSTT1 polymorphisms are not associated with gastric cancer in a small group of the Turkish population.
谷胱甘肽S-转移酶（GST）是催化包括致癌物在内的反应的酶。GSTT1和GSTM1基因在人类中具有多态性。一些GST基因多态性与癌症之间的关系已有报道。在本研究中，我们旨在调查一组胃癌患者中GSTT1和GSTM1多态性的分布情况。研究组由来自一家培训医院病理科档案中的50例胃腺癌患者（21名女性，29名男性）组成。57名健康对照者作为对照组纳入研究。从对照者的外周静脉血和病例的石蜡块中提取DNA。采用双重聚合酶链反应对GSTT1和GSTM1基因进行基因分型。在患者和健康受试者之间未观察到GSTM1或GSTT1无效基因型频率的差异（GSTM1：52%对43.85%，OR = 1.27，95%CI：0.55 - 2.96；GSTT1：38.46%对28.07%，OR = 0.72，95%CI：0.25 - 1.96）。此外，两组中两种基因型的同时携带情况几乎相同。在弥漫型或肠型胃癌中，GSTM1或GSTT1无效基因型并无差异。我们的数据表明，在一小部分土耳其人群中，GSTM1和GSTT1多态性与胃癌无关。
