Mosayebi Ghasem, Alizadeh Shaban Ali, Alasti Ali, Amouzandeh Nobaveh Alireza, Ghazavi Ali, Okhovat Mahsa, Rafiei Mohammad
Department of Immunology and Microbiology, School of Medicine, Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran, e-mail:
Iran J Immunol. 2013 Dec;10(4):216-28.
The appendix is considered as part of the gut-associated lymphoid tissue; however, lymphocyte subsets in this tissue are not fully defined.
To investigate and compare the function and phenotype of lymphocyte subsets in peripheral blood and appendix of patients with normal and inflamed appendix tissues.
Peripheral blood samples and appendiceal mononuclear cells were obtained from 81 patients (mean age; 23 ± 10.5 years), clinically suspected of having appendicitis. The phenotypic characteristics of lymphocyte subsets in peripheral blood (before and 48-72 hrs after appendectomy) and in appendix tissue were analyzed by three color-flow cytometry. The proliferative response of mononuclear cells was assessed by MTT method.
The frequency of CD19+DR+, HLA-DR+ and CD19+ cells in the appendix tissue were significantly higher than that of the peripheral blood in all the groups (p<0.001). The percentage of CD19+ cells and HLA-DR+CD19+ cells significantly decreased after appendectomy in the peripheral blood of the patients with acute appendicitis (p=0.047 and p=0.03, respectively). CD19 and HLA-DR plus CD19 had better diagnostic efficiency compared with T cell markers (area under the ROC curve [AUC]= 0.76 and 0.73, respectively).
These results indicate a significant difference in CD19+ and HLA-DR+ lymphocytes between peripheral blood and the appendix tissue.
阑尾被认为是肠道相关淋巴组织的一部分；然而，该组织中的淋巴细胞亚群尚未完全明确。
研究并比较正常阑尾组织和发炎阑尾组织患者外周血及阑尾中淋巴细胞亚群的功能和表型。
从81例临床疑似阑尾炎患者（平均年龄23±10.5岁）获取外周血样本和阑尾单个核细胞。通过三色流式细胞术分析外周血（阑尾切除术前及术后48 - 72小时）和阑尾组织中淋巴细胞亚群的表型特征。采用MTT法评估单个核细胞的增殖反应。
所有组中，阑尾组织中CD19+DR+、HLA - DR+及CD19+细胞的频率显著高于外周血（p<0.001）。急性阑尾炎患者外周血中，阑尾切除术后CD19+细胞及HLA - DR+CD19+细胞的百分比显著降低（分别为p = 0.047和p = 0.03）。与T细胞标志物相比，CD19以及HLA - DR加CD19具有更好的诊断效率（ROC曲线下面积[AUC]分别为0.76和0.73）。
这些结果表明外周血和阑尾组织中CD19+和HLA - DR+淋巴细胞存在显著差异。
