Beaudoin A R, St-Jean P, Vachereau A
Pancreas. 1986;1(1):2-4. doi: 10.1097/00006676-198601000-00002.
By pulse and chase experiments with [3H]leucine we have followed the time-course packaging of newly synthesized amylase in the zymogen granules of pig pancreas in vivo. In parallel, with a second group of pigs, we have followed the time-course release of newly synthesized amylase. The ratios of specific radioactivities of amylase in the zymogen granule over that of the homogenate indicated that substantial amounts of newly synthesized amylase had already reached the zymogen granule compartment at 50-min postpulse. Specific radioactivity increased up to 180 min, then a plateau was obtained. In the juice with the other group of pigs, starting at 40 min postpulse, amylase specific radioactivity increased up to about 100 min, then slowly decreased in the next hours. These results show an asynchronism between packaging of newly synthesized amylase in the zymogen granule compartment and its release in the juice under resting conditions, supporting the concept of a partial bypass of the zymogen granule compartment.
通过用[3H]亮氨酸进行脉冲追踪实验，我们在体内追踪了猪胰腺中酶原颗粒中新合成淀粉酶的包装时间进程。同时，对于另一组猪，我们追踪了新合成淀粉酶的释放时间进程。酶原颗粒中淀粉酶的比放射性与匀浆中淀粉酶的比放射性之比表明，在脉冲后50分钟时，大量新合成的淀粉酶已经到达酶原颗粒区室。比放射性在180分钟前持续增加，然后达到平稳状态。在另一组猪的胰液中，从脉冲后40分钟开始，淀粉酶比放射性在约100分钟前增加，然后在接下来的几个小时内缓慢下降。这些结果表明，在静息条件下，新合成淀粉酶在酶原颗粒区室的包装与其在胰液中的释放之间存在异步性，支持了酶原颗粒区室部分旁路的概念。
