Henrion R, Papa F, Moreau F, Jardel-Guermeur C, Yonger J, Ekindjian O G
Rev Fr Gynecol Obstet. 1987 Jan;82(1):19-22.
The authors report the results of 434 titrations of acetylcholinesterase in the amniotic fluid sampled by early amniocentesis, between the 17th and 24th week of amenorrhea. The biochemical technique used was quantitative and the values of the total cholinesterase activity (TchE) and the true acetylcholinesterase (AchE) were studied separately. Their conclusion is similar to that of other authors: the quantitative method, although satisfactory, has less value than the qualitative method using electrophoresis on polyacrylamide gel in order to screen or confirm the existence of a dysraphia of the central nervous system, because any amount of soiled fetal blood elevates the acetylcholinesterase level, titrated by the quantitative method.
作者报告了在闭经第17至24周期间通过早期羊膜腔穿刺术采集的羊水样本中434次乙酰胆碱酯酶滴定的结果。所使用的生化技术是定量的，分别研究了总胆碱酯酶活性（TchE）和真性乙酰胆碱酯酶（AchE）的值。他们的结论与其他作者相似：定量方法虽然令人满意，但与使用聚丙烯酰胺凝胶电泳的定性方法相比，其价值较低，后者用于筛查或确认中枢神经系统神经管缺陷的存在，因为任何量的污染胎儿血液都会提高通过定量方法滴定的乙酰胆碱酯酶水平。
