汽车尾气颗粒物对哺乳动物组织培养细胞的细胞活力、接种效率和细胞分裂的影响。

The effect of automobile exhaust particulates on cell viability, plating efficiency and cell division of mammalian tissue culture cells.

作者信息

Seemayer N H, Hadnagy W, Tomingas R

出版信息

Sci Total Environ. 1987 Mar;61:107-15. doi: 10.1016/0048-9697(87)90360-3.

Abstract

Extract of particulate matter (EPM) of gasoline engine exhaust induced only a slight loss of cell viability of mouse macrophages (line IC-21) in vitro, while a strong dose-dependent reduction of plating efficiency of human cell line A-549 and of Syrian hamster line 14-1b occurred. Cytological investigations of exposed macrophages of line IC-21 revealed an increase in the mitotic index from 1.5% of control values up to 14.6% at the highest tested concentration of EPM. Mitotic arrest is based almost exclusively on C-type mitoses occurring dose-dependently in the presence of EPM. Results indicate disturbances of the spindle apparatus in the presence of EPM.

摘要

汽油发动机尾气颗粒物提取物（EPM）在体外仅引起小鼠巨噬细胞（IC-21系）细胞活力轻微丧失，而人A-549细胞系和叙利亚仓鼠14-1b细胞系的接种效率则出现强烈的剂量依赖性降低。对IC-21系暴露巨噬细胞的细胞学研究显示，在最高测试浓度的EPM作用下，有丝分裂指数从对照值的1.5%增加到了14.6%。有丝分裂停滞几乎完全基于在EPM存在下剂量依赖性出现的C型有丝分裂。结果表明在EPM存在下纺锤体装置受到干扰。