Parry E M, Hague A, Parry J M
School of Biological Sciences, University College of Swansea, U.K.
Mutat Res. 1990 Mar;237(2):83-93. doi: 10.1016/0921-8734(90)90014-i.
Events associated with culture ageing in Syrian hamster dermal cells have been studied from the time of culture isolation during continuous passage until they senesced and died. Microscopic examination of mitotic cells using differential staining of chromosome and spindle apparatus assessed the faithfulness of cell division. Other indicators of the quality of cell division were obtained from chromosome counts, micronucleus frequencies and incidences of binucleate cells. A loss of spindle fidelity and an increase in aneuploidy corresponded to the period of culture senescence. The data presented indicate that the loss of division fidelity and chromosome number instability is an important indicator of the progression of a mammalian culture to senescence under in vitro conditions. Such information may provide the basis of a model for the study of factors which modify mitotic fidelity and senescence and provide a methodology for monitoring the suitability of mammalian cultures for commercial usage.
从叙利亚仓鼠真皮细胞培养分离开始，在连续传代直至衰老死亡的过程中，对与细胞培养衰老相关的事件进行了研究。使用染色体和纺锤体装置的差异染色对有丝分裂细胞进行显微镜检查，评估细胞分裂的忠实性。细胞分裂质量的其他指标来自染色体计数、微核频率和双核细胞发生率。纺锤体保真度的丧失和非整倍体的增加与培养衰老期相对应。所呈现的数据表明，分裂保真度的丧失和染色体数目不稳定性是体外条件下哺乳动物培养物向衰老进展的重要指标。这些信息可能为研究改变有丝分裂保真度和衰老的因素的模型提供基础，并为监测哺乳动物培养物用于商业用途的适用性提供一种方法。
