Bouda J, Jagos P, Klimes J, Minksová E, Jonáková V
Vet Med (Praha). 1987 Mar;32(3):135-44.
The activity of the colostral trypsin inhibitor (CTI) was studied in seven milkings of colostrum in the course of the first five days after calving in 15 cows. The activity of the total trypsin inhibitor in the colostrum was ascertained in the course of the first two days after calving in ten cows in their second to fourth lactations and also in the colostrum of the first milking after calving in the case of 11 cows. The greatest CTI activity was found in the colostrum of the first milking after calving (301 micrograms X ml-1). Between the CTI activity and the concentration of total protein (CB) in the whey of the colostrum from the first and second day after calving there was found to be a statistically significant correlation (r = +0.929). The activity of total trypsin inhibitor was also highest in the colostrum from the first milking (499 micrograms X ml-1). After calving the CTI and the total trypsin inhibitor activities in the colostrum gradually dropped. Between the activity of the total trypsin inhibitor and the CB concentration in whey from the first, third, fifth and seventh milkings after birth a statistically significant correlation was found (r = +0.972). Statistically significant correlations were also found between the activity of the total trypsin inhibitor, the specific gravity of colostrum, the concentration of total protein and gamma-globulins in the whey of the colostrum from the first milking after calving. In metabolic alkalosis the concentration of total protein in the whey and the activity of trypsin inhibitor were found to be considerably lower than the average values.
对15头母牛产后头五天内七次初乳挤奶中的初乳胰蛋白酶抑制剂（CTI）活性进行了研究。在10头处于第二至第四泌乳期的母牛产后头两天内，以及11头母牛产后首次挤奶的初乳中，测定了初乳中总胰蛋白酶抑制剂的活性。发现产后首次挤奶的初乳中CTI活性最高（301微克×毫升-1）。在产后第一天和第二天初乳乳清中，CTI活性与总蛋白（CB）浓度之间存在统计学上的显著相关性（r = +0.929）。首次挤奶的初乳中总胰蛋白酶抑制剂活性也最高（499微克×毫升-1）。产后初乳中CTI和总胰蛋白酶抑制剂活性逐渐下降。在出生后首次、第三次、第五次和第七次挤奶的初乳乳清中，总胰蛋白酶抑制剂活性与CB浓度之间存在统计学上的显著相关性（r = +0.972）。在产后首次挤奶的初乳中，总胰蛋白酶抑制剂活性、初乳比重、乳清中总蛋白和γ-球蛋白浓度之间也存在统计学上的显著相关性。在代谢性碱中毒中，发现乳清中总蛋白浓度和胰蛋白酶抑制剂活性明显低于平均值。
