一种源自患有肺腺癌（南非绵羊肺腺瘤病）的南非绵羊的新型慢病毒的特征。

Characteristics of a novel lentivirus derived from South African sheep with pulmonary adenocarcinoma (jaagsiekte).

作者信息

Quérat G, Barban V, Sauze N, Vigne R, Payne A, York D, De Villiers E M, Verwoerd D W

出版信息

Virology. 1987 May;158(1):158-67. doi: 10.1016/0042-6822(87)90249-2.

Abstract

A novel lentivirus was isolated from South African sheep with experimentally transmitted lung adenocarcinoma. Similar to visna virus and caprine arthritis encephalitis virus, this new strain induced cytopathic effects on ovine plexus choroid cultures. In contrast to a recent Israeli isolate from sheep with adenocarcinoma, the South African lentivirus could not transform fibroblast cultures. The antigenic relatedness between the new isolate and visna virus was assessed by immunoprecipitation of radiolabeled viral proteins, using monospecific antisera against visna virus proteins. The results indicate that the new virus contains four major structural proteins of sizes similar to those of visna virus (i.e., gp135, p30, p16, and p14) and have some common antigenic determinants (about 90% in the major core antigen p30). However, the nucleotidic sequences of the novel lentivirus were found to be only 16.5 to 27.4% homologous to visna virus and 8.3 to 15% homologous to caprine arthritis encephalitis virus, by means of liquid hybridization under stringent conditions. The genetic divergence indicated by this last result was confirmed by the dissimilar restriction endonuclease cleavage map of the new virus in comparison to those of visna virus and three caprine arthritis encephalitis virus strains. The demonstration of a third type of ovine lentivirus supports the concept of an important genetic variation among the lentiviruses infecting one animal species.

摘要

从实验性传播肺腺癌的南非绵羊中分离出一种新型慢病毒。与维斯纳病毒和山羊关节炎脑炎病毒相似，这种新毒株对绵羊脉络丛培养物产生细胞病变效应。与最近从患腺癌的以色列绵羊中分离出的毒株不同，南非慢病毒不能转化成纤维细胞培养物。使用针对维斯纳病毒蛋白的单特异性抗血清，通过对放射性标记病毒蛋白进行免疫沉淀，评估新分离毒株与维斯纳病毒之间的抗原相关性。结果表明，新病毒含有四种主要结构蛋白，其大小与维斯纳病毒的相似（即gp135、p30、p16和p14），并且有一些共同的抗原决定簇（在主要核心抗原p30中约为90%）。然而，通过在严格条件下的液相杂交发现，该新型慢病毒的核苷酸序列与维斯纳病毒的同源性仅为16.5%至27.4%，与山羊关节炎脑炎病毒的同源性为8.3%至15%。与维斯纳病毒和三种山羊关节炎脑炎病毒毒株相比，新病毒不同的限制性内切酶切割图谱证实了最后这一结果所表明的基因差异。第三种绵羊慢病毒的证明支持了感染同一动物物种的慢病毒之间存在重要基因变异的观点。