山羊和绵羊慢病毒dUTPase缺陷型突变体的复制特性
Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses.
作者信息
Turelli P, Pétursson G, Guiguen F, Mornex J F, Vigne R, Quérat G
机构信息
INSERM U372, Marseille, France.
出版信息
J Virol. 1996 Feb;70(2):1213-7. doi: 10.1128/JVI.70.2.1213-1217.1996.
Abstract
The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measure the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular clone from the Cork isolate was dUTPase defective as a result of a single amino acid substitution. Using this point mutant and deletion mutants of CAEV as well as a deletion mutant of visna virus, we demonstrated that dUTPase-deficient viruses replicate similarly to wild-type viruses in dividing cells but show delayed replication in nondividing primary macrophages.
摘要
利用一种测定方法来确定山羊关节炎-脑炎病毒（CAEV）和维斯纳病毒的病毒体相关dUTP酶活性，该方法可测量dUTP酶在逆转录过程中防止dUTP错误掺入cDNA的实际能力。我们发现，来自科克分离株的CAEV分子克隆由于单个氨基酸取代而存在dUTP酶缺陷。利用该点突变体和CAEV的缺失突变体以及维斯纳病毒的缺失突变体，我们证明dUTP酶缺陷型病毒在分裂细胞中的复制与野生型病毒相似，但在非分裂的原代巨噬细胞中显示出延迟复制。
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