台湾地区上尿路尿路上皮癌中谷胱甘肽S-转移酶的表达研究
Glutathione S-transferase expression in upper urinary tract urothelial carcinomas: a Taiwan study.
作者信息
Chen Szu-Han, Wu Wen-Jeng, Tu Hung-Pin, Li Wei-Ming, Huang Chun-Nung, Li Ching-Chia, Lin Hui-Hui, Ke Hung-Lung
机构信息
Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan E-mail :
出版信息
Asian Pac J Cancer Prev. 2013;14(11):6475-9. doi: 10.7314/apjcp.2013.14.11.6475.
OBJECTIVES
Glutathione S-transferase (GST) isoenzymes play important roles in resistance to cell apoptosis and carcinogenesis. We aimed to establish the relationship between GST expression and the prognosis of upper urinary tract urothelial carcinoma (UTT-UC) in Taiwan.
METHODS
This study retrospectively reviewed 46 patients with pathologically confirmed UUT-UC at Kaohsiung Medical University Hospital. In each patient, expression of GSTT1 and GSTP1 was compared between urothelial carcinoma and normal urothelial cells by Western blotting.
RESULTS
GSTP1 expression in the UUT-UC cells was significantly higher than that in normal urothelial cells (1.6 fold, p<0.001). Expression of GSTT1 was significantly associated with the invasiveness of the carcinoma (p=0.006).
CONCLUSIONS
In UUT-UC, GSTP1 might be a potential tumor marker, whereas high GSTT1 expression could be used as an indicator of cancer progression. This study is the first to demonstrate potential applications of different GST isoenzymes for biomolecular analysis of UUT-UCs in Taiwan.
目的
谷胱甘肽S-转移酶（GST）同工酶在抵抗细胞凋亡和致癌作用中发挥重要作用。我们旨在建立台湾地区上尿路尿路上皮癌（UTT-UC）中GST表达与预后之间的关系。
方法
本研究回顾性分析了高雄医学大学医院46例经病理确诊的上尿路尿路上皮癌患者。通过蛋白质印迹法比较了每位患者尿路上皮癌细胞与正常尿路上皮细胞中GSTT1和GSTP1的表达。
结果
上尿路尿路上皮癌细胞中GSTP1的表达显著高于正常尿路上皮细胞（1.6倍，p<0.001）。GSTT1的表达与癌的侵袭性显著相关（p=0.006）。
结论
在上尿路尿路上皮癌中，GSTP1可能是一种潜在的肿瘤标志物，而高GSTT1表达可作为癌症进展的指标。本研究首次证明了不同GST同工酶在台湾地区上尿路尿路上皮癌生物分子分析中的潜在应用。
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