Natphopsuk Sitakan, Settheetham-Ishida Wannapa, Pientong Chamsai, Sinawat Supat, Yuenyao Pissamai, Ishida Takafumi, Settheetham Dariwan
Department of Physiology, Faculty of Public Health, Khon Kaen University, Khon Kaen, Thailand E-mail :
Asian Pac J Cancer Prev. 2013;14(11):6961-4. doi: 10.7314/apjcp.2013.14.11.6961.
Human papillomavirus (HPV) is a major cause of cervical cancer. More than 100 HPV genotypes have been identified; however the distribution varies geographically and according to ethnicity. The purpose of this study was to investigate the prevalence and distribution of HPV subtypes among Northeast Thai women. Subjects included 198 cases of SCCA and 198 age-matched, healthy controls. HPV-DNA was amplified by PCR using the consensus primers GP5+/6+ system followed by reverse line blot hybridization genotyping. The prevalence of high-risk HPV infection was 21 (10.1%) and 152 (76.8%) in the controls and in the cases, respectively. High-risk HPV significantly increased the risk for cervical cancer with an OR of 42.4 (95%CI: 22.4-81.4, p<0.001) and an adjusted OR of 40.7-fold (95%CI: 21.5-76.8, p <0.001). HPV-16 was the most prevalent HPV type in the SCCA (56.2%) followed by HPV-58 (17.8%) and HPV-18 (13.6%); whereas HPV-58 (46.4%) was a prominent genotype in the controls followed by HPV-16 (39.3%) and unidentified HPV types (25.0%). These findings indicate that HPV infection remains a critical risk factor for SCCA; particularly, HPV-16, HPV-58 and HPV-18. In order to eradicate cervical cancer, sustained health education, promoted use of prophylactics and a HPV-58 vaccine should be introduced in this region.
人乳头瘤病毒（HPV）是宫颈癌的主要病因。已鉴定出100多种HPV基因型；然而，其分布因地理位置和种族而异。本研究的目的是调查泰国东北部女性中HPV亚型的流行情况和分布。研究对象包括198例鳞状细胞癌（SCCA）患者和198例年龄匹配的健康对照者。使用通用引物GP5+/6+系统通过聚合酶链反应（PCR）扩增HPV-DNA，随后进行反向线印迹杂交基因分型。高危HPV感染在对照组和病例组中的患病率分别为21例（10.1%）和152例（76.8%）。高危HPV显著增加了患宫颈癌的风险，比值比（OR）为42.4（95%置信区间：22.4 - 81.4，p<0.001），调整后的OR为40.7倍（95%置信区间：21.5 - 76.8，p <0.001）。HPV-16是SCCA中最常见的HPV类型（56.2%），其次是HPV-58（17.8%）和HPV-18（13.6%）；而HPV-58（46.4%）是对照组中突出的基因型，其次是HPV-16（39.3%）和未鉴定的HPV类型（25.0%）。这些发现表明，HPV感染仍然是SCCA的关键危险因素；特别是HPV-16、HPV-58和HPV-18。为了根除宫颈癌，该地区应持续开展健康教育，推广使用预防性措施，并引入HPV-58疫苗。
