Department of Obstetrics and Gynecology Clinic III, Izmir Ataturk Training and Research Hospital, Izmir, Turkey.
Eur J Obstet Gynecol Reprod Biol. 2011 Nov;159(1):168-71. doi: 10.1016/j.ejogrb.2011.06.021. Epub 2011 Jul 18.
This study aimed to detect the presence and prevalence of HPV-DNA in the cervical swab samples obtained from patients with cervical cancer, premalignant cervical lesions and benign cervical smear results, and to identify the potential risk factors influencing this prevalence.
Smear preparations were examined and classified according to the Bethesda system. HPV-DNA detection and genotyping was carried out using polymerase chain reaction combined with reverse hybridization line-probe assays. Age, smoking habit, age at first sexual intercourse, number of sexual partners, number of term births, contraceptive method, progesterone therapy, history of sexually transmitted diseases, history or existence of warts, existence of cervical infection and the history of circumcision of male sexual partners were recorded.
Six hundred and forty-two women (96 women with abnormal cervical cytology and 546 women with normal cytology) provided cervical samples. Multiplex PCR testing revealed that prevalence of HPV-DNA was 38.9% in our study population. HPV-DNA was detected in 78.3% of the women with cervical cancer and 76.9% of the women with HGSIL. Abnormal cervical cytology was observed in 30% of HPV-DNA positive cases and in 5.4% of HPV-DNA negative cases. Our findings also indicate that smoking habit, number of sexual partners, history of sexually transmitted diseases, and abnormal cervical cytology were associated with HPV infection. With respect to parity, there was a decreased risk of HPV infection with the increase in the number of births.
Estimates of the prevalence of HPV infection vary greatly around the world, so the factors that contribute to the rare occurrence of cervical cancer after HPV infection might also differ from country to country. Information gathered from this study could be used to prioritize limited screening and treatment services given to woman who have specific characteristics that may put them at an increased risk of HPV disease.
本研究旨在检测宫颈癌、癌前宫颈病变和良性宫颈涂片患者宫颈拭子样本中 HPV-DNA 的存在和流行情况，并确定影响这种流行率的潜在危险因素。
根据巴氏系统对涂片进行检查和分类。采用聚合酶链反应结合反向杂交线探针分析进行 HPV-DNA 检测和基因分型。记录年龄、吸烟习惯、初次性行为年龄、性伴侣数量、足月分娩次数、避孕方法、孕激素治疗、性传播疾病史、疣病史或存在、宫颈感染史和男性性伴侣的割礼史。
642 名女性（96 名宫颈细胞学异常女性和 546 名细胞学正常女性）提供了宫颈样本。多重 PCR 检测显示，本研究人群中 HPV-DNA 的流行率为 38.9%。HPV-DNA 在宫颈癌妇女中检出率为 78.3%，在 HSIL 妇女中检出率为 76.9%。异常宫颈细胞学在 HPV-DNA 阳性病例中的检出率为 30%，在 HPV-DNA 阴性病例中的检出率为 5.4%。我们的研究结果还表明，吸烟习惯、性伴侣数量、性传播疾病史和异常宫颈细胞学与 HPV 感染有关。就产次而言，随着分娩次数的增加，HPV 感染的风险降低。
HPV 感染的流行率在世界各地差异很大，因此导致 HPV 感染后宫颈癌罕见发生的因素也可能因国家而异。从本研究中收集的信息可用于优先考虑对具有特定特征的女性进行有限的筛查和治疗服务，这些特征可能使她们面临更高的 HPV 疾病风险。
