一种无需使用核糖核酸酶大规模分离无RNA质粒和噬菌体DNA的方法。
A procedure for large-scale isolation of RNA-free plasmid and phage DNA without the use of RNase.
作者信息
Lev Z
出版信息
Anal Biochem. 1987 Feb 1;160(2):332-6. doi: 10.1016/0003-2697(87)90055-8.
PMID:2437820
Abstract
A preparative procedure for the large-scale isolation of plasmid DNA without the use of RNAse is described. Crude plasmid DNA is prepared using a standard boiling method. High-molecular-weight RNA is removed by precipitation with LiCl, and low-molecular-weight RNA is removed by sedimentation through high-salt solution. The procedure is inexpensive, rapid, simple, and particularly suitable for processing several large-scale preparations simultaneously. A similar procedure has been developed for preparation of lambda-phage DNA.
摘要
本文描述了一种不使用RNA酶大规模分离质粒DNA的制备方法。粗制质粒DNA采用标准煮沸法制备。通过用LiCl沉淀去除高分子量RNA，通过高盐溶液沉降去除低分子量RNA。该方法成本低、速度快、操作简单，特别适合同时处理多个大规模制备物。已开发出一种类似的方法用于制备λ噬菌体DNA。
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