Lev Z
Anal Biochem. 1987 Feb 1;160(2):332-6. doi: 10.1016/0003-2697(87)90055-8.
A preparative procedure for the large-scale isolation of plasmid DNA without the use of RNAse is described. Crude plasmid DNA is prepared using a standard boiling method. High-molecular-weight RNA is removed by precipitation with LiCl, and low-molecular-weight RNA is removed by sedimentation through high-salt solution. The procedure is inexpensive, rapid, simple, and particularly suitable for processing several large-scale preparations simultaneously. A similar procedure has been developed for preparation of lambda-phage DNA.
本文描述了一种不使用RNA酶大规模分离质粒DNA的制备方法。粗制质粒DNA采用标准煮沸法制备。通过用LiCl沉淀去除高分子量RNA，通过高盐溶液沉降去除低分子量RNA。该方法成本低、速度快、操作简单，特别适合同时处理多个大规模制备物。已开发出一种类似的方法用于制备λ噬菌体DNA。
