Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan.
Sensors (Basel). 2013 Dec 27;14(1):458-67. doi: 10.3390/s140100458.
A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successfully demonstrated to have the same spectral half-width in the dip for optimum coupling. The sensitivity was shown to be -0.087 dB/(μg/mL) at all applied telecommunication wavelengths and the highest sensitivity achieved was 115 ng/mL without thiolated DNA immobilization onto a gold plate, which is better than the sensor limit of 400 ng/mL possible with commercial biosensor equipment.
一种基于光纤通信激光波长和稳定性的可重复电信波长调制的结核分枝杆菌（MTB）脱氧核糖核酸（DNA）表面等离子体共振传感器被开发出来。成功地证明了 1μg/mL 和 10μg/mL 的 MTB DNA 浓度在最佳耦合时具有相同的光谱半宽度。在所有应用的电信波长下，灵敏度显示为-0.087 dB/（μg/mL），而在不将巯基化 DNA固定到金板上的情况下，最高灵敏度达到 115ng/mL，优于商用生物传感器设备可能达到的传感器极限 400ng/mL。
