Allen C N, Albuquerque E X
Brain Res. 1987 Apr 28;410(1):159-63. doi: 10.1016/s0006-8993(87)80039-2.
The conductance characteristics of gamma-aminobutyric acid-activated single channel currents from cultured hippocampal neurons were examined using patch clamp techniques. GABA-activated currents had amplitudes which were linearly correlated to the membrane potentials over a range of -80 to +70 mV and an open time and burst time of 2.2 and 4.3 ms, respectively. The conductance of the gamma-aminobutyric acid-activated channels was 19 pS. These data demonstrate that cultured hippocampal neurons have channel conductances which have characteristics different from those of adult neurons.
运用膜片钳技术检测了培养的海马神经元中γ-氨基丁酸激活的单通道电流的电导特性。γ-氨基丁酸激活的电流幅度在-80至+70 mV范围内与膜电位呈线性相关，开放时间和爆发时间分别为2.2毫秒和4.3毫秒。γ-氨基丁酸激活通道的电导为19皮西门子。这些数据表明，培养的海马神经元具有与成年神经元不同的通道电导特性。
