兔迷走神经轴突运输慢相中与钙调蛋白结合的蛋白质。

Calmodulin-binding proteins within the slow phase of axonal transport in the rabbit vagus nerve.

作者信息

Ekström P, Kanje M

出版信息

J Neurochem. 1987 Jul;49(1):146-51. doi: 10.1111/j.1471-4159.1987.tb03406.x.

DOI:10.1111/j.1471-4159.1987.tb03406.x

Abstract

Calmodulin-binding proteins (CBPs) in the rabbit vagus nerve were studied by means of calmodulin-Sepharose affinity chromatography and polyacrylamide gel electrophoresis. The soluble fraction (10(5) g supernatant) of a nerve homogenate contained four CBPs with molecular weights of 44, 55, 91, and 93 kD, respectively. Slowly transported proteins were recovered in the vagus 3 days after injection of [35S]methionine into the nodose ganglion. Four labelled CBPs with molecular weights of 44, 55, 69, and 83 kD, respectively were found. The nodose ganglion contained two labelled CBPs, 44 and 55 kD. The 55-kD CBP was identified as tubulin after immunoblotting. In separate experiments it was also shown that bovine brain tubulin bound to the calmodulin column.

摘要

采用钙调蛋白-琼脂糖亲和层析法和聚丙烯酰胺凝胶电泳法对兔迷走神经中的钙调蛋白结合蛋白（CBP）进行了研究。神经匀浆的可溶性部分（10⁵g上清液）含有四种CBP，分子量分别为44、55、91和93kD。向结状神经节注射[³⁵S]甲硫氨酸3天后，在迷走神经中回收了缓慢转运的蛋白质。发现了四种分别具有44、55、69和83kD分子量的标记CBP。结状神经节含有两种标记CBP，44kD和55kD。免疫印迹后，55kD的CBP被鉴定为微管蛋白。在单独的实验中还表明，牛脑微管蛋白与钙调蛋白柱结合。