Pappius H M, Dadoun R
J Neurochem. 1987 Jul;49(1):321-5. doi: 10.1111/j.1471-4159.1987.tb03433.x.
It was shown previously that focal cortical freezing lesions in rats cause widespread depression of local cerebral glucose utilization (LCGU) in cortical areas of the lesioned hemisphere. This was interpreted as reflecting functional depression. The underlying mechanisms were postulated to involve alterations of biogenic amine systems. Accordingly, levels of serotonin (5-HT), its metabolite 5-hydroxyindoleacetic acid (5-HIAA), and its precursor tryptophan were determined by an HPLC method with electrochemical detection in frontoparietal cortical areas of both hemispheres at 4 h and 1, 3, 6, 8, and 10 days after a unilateral cortical freezing lesion. The 5-HT content was significantly lower than normal in the lesioned hemisphere only at 24 h, whereas the 5-HIAA level peaked at 24 h but was significantly elevated above normal values between 4 h and 6 days after lesioning. No changes were noted in 5-HT and 5-HIAA contents in the hemisphere contralateral to the lesion. These results indicate that cortical 5-HT metabolism is increased throughout the lesioned hemisphere of a focally injured brain. The increase in tryptophan content of the lesioned brain appeared to have a time course more closely related to previously demonstrated changes in cortical LCGU than to the increase in 5-HIAA content.
先前的研究表明，大鼠局灶性皮质冷冻损伤会导致损伤半球皮质区域局部脑葡萄糖利用（LCGU）广泛降低。这被解释为反映了功能抑制。据推测，其潜在机制涉及生物胺系统的改变。因此，采用高效液相色谱法结合电化学检测，在单侧皮质冷冻损伤后4小时以及1、3、6、8和10天，测定双侧半球额顶叶皮质区域中血清素（5-HT）、其代谢产物5-羟吲哚乙酸（5-HIAA）及其前体色氨酸的水平。仅在24小时时，损伤半球的5-HT含量显著低于正常水平，而5-HIAA水平在24小时达到峰值，但在损伤后4小时至6天显著高于正常值。损伤对侧半球的5-HT和5-HIAA含量未发现变化。这些结果表明，在局灶性损伤脑的整个损伤半球中，皮质5-HT代谢增加。损伤脑内色氨酸含量的增加，其时间进程似乎与先前证明的皮质LCGU变化的关系比与5-HIAA含量增加的关系更为密切。
