The macroglial cells of the leech are molecularly heterogeneous.

Monoclonal antibodies derived from fusions employing either whole leech nerve cords or fractionated proteins (gel bands) bind to the macroglial cells of the nerve cord. Three different antibodies bind to either one, two, or three of the four macroglial cell types in the leech CNS, serving as markers and showing that these four cell types, which differ primarily by anatomical position, all differ molecularly as well. Conventional microscopy confirms the existence of a novel macroglial cell type first noted because it binds antibody. Western (immunoblot) blot analyses demonstrate a polypeptide antigen of 77 kD in the macroglial cells of the connectives and a 130-kD polypeptide antigen associated with the macroglial cells of the connectives, of the root nerves, and of the ganglionic neuropil. The extensive molecular heterogeneity of leech neurons demonstrated by monoclonal antibody techniques is shared by macroglia.