Li C-L, Yang X-L, Wang J-J, Du G-H, Yang W-M, Zhang H-P
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Andrologia. 2015 Feb;47(1):25-9. doi: 10.1111/and.12217. Epub 2014 Jan 6.
This study aimed to explore the effects of intracavernous injection (ICI) of P2X3 and NK1 receptor antagonists on erectile dysfunction (ED) induced by spinal cord transection in rats. Sixty male Sprague-Dawley (SD) rats were randomly divided into the following three groups (20 rats each group): sham operation group (C group), thoracic spinal cord transection group (T group) and sacral spinal cord transection group (S group). An ED model was established through complete transection of the thoracic or sacral spinal cord. Intracavernous pressure (ICP) with and without injection of P2X3 (Suramin) or NK1 (GR82334) receptor antagonists was recorded 3 weeks after surgery. Immunohistochemistry was employed to detect the expression of P2X3 and NK1 receptors in the dorsal root ganglion (DRG) and smooth muscle of corpus cavernosum. Data were processed with SPSS 17.0. ICI with Suramin (0.1, 0.3 and 1 mm) or GR82334 (0.1, 0.3 and 1 mm) increased ICP dose dependently in the T and S groups. The expression of P2X3 and NK1 receptors in DRG and smooth muscle of corpus cavernosum was up-regulated in the T and S groups. It is concluded that ICI of P2X3 and NK1 receptor antagonists may improve the recovery of erectile function in a rat model with ED after spinal cord transection.
本研究旨在探讨海绵体内注射P2X3和NK1受体拮抗剂对大鼠脊髓横断所致勃起功能障碍（ED）的影响。将60只雄性Sprague-Dawley（SD）大鼠随机分为以下三组（每组20只）：假手术组（C组）、胸段脊髓横断组（T组）和骶段脊髓横断组（S组）。通过完全横断胸段或骶段脊髓建立ED模型。术后3周记录注射P2X3（苏拉明）或NK1（GR82334）受体拮抗剂前后的海绵体内压（ICP）。采用免疫组织化学法检测背根神经节（DRG）和海绵体平滑肌中P2X3和NK1受体的表达。数据用SPSS 17.0处理。在T组和S组中，注射苏拉明（0.1、0.3和1 mM）或GR82334（0.1、0.3和1 mM）可使ICP剂量依赖性增加。T组和S组中DRG和海绵体平滑肌中P2X3和NK1受体的表达上调。结论是，P2X3和NK1受体拮抗剂的海绵体内注射可能改善脊髓横断后ED大鼠模型勃起功能的恢复。
