Roellinger S, Loos M, Leibold W
Scand J Immunol. 1987 May;25(5):507-15. doi: 10.1111/j.1365-3083.1987.tb02222.x.
A high molecular polyanion, Liquoid, was found to inhibit at nontoxic concentrations (12-50 micrograms/ml) the natural killing (NK) and the antibody-dependent cellular cytotoxic (ADCC) activity of human peripheral blood mononuclear cells selectively. Whereas NK of the K 562 target cell was slightly or not at all affected, the spontaneous lysis of PDe-B-1, an EBV-transformed B-cell line, was strongly inhibited or even completely abolished. ADCC activity could only be inhibited by Liquoid if the target cells were mycoplasma-free, while the polyanion had no effect when mycoplasma-contaminated target cells were used. Liquoid did not alter the target binding capacity of the NK effector cells and did not activate monocytes or induce other suppressive cells. Alpha interferon, but neither beta nor gamma interferon, was able to neutralize the NK reduction. These results suggest that Liquoid inhibits a target cell-related, selective process in the post-binding stage of NK cell lysis.
发现一种高分子量的聚阴离子——多乙烯磺酸钠，能在无毒浓度（12 - 50微克/毫升）下选择性抑制人外周血单个核细胞的自然杀伤（NK）和抗体依赖性细胞毒性（ADCC）活性。K 562靶细胞的NK活性略有影响或根本未受影响，而EB病毒转化的B细胞系PDe - B - 1的自发裂解则受到强烈抑制甚至完全消除。只有当靶细胞无支原体时，多乙烯磺酸钠才能抑制ADCC活性，而使用受支原体污染的靶细胞时，该聚阴离子则无作用。多乙烯磺酸钠不会改变NK效应细胞的靶细胞结合能力，也不会激活单核细胞或诱导其他抑制性细胞。α干扰素，但不是β或γ干扰素，能够中和NK活性的降低。这些结果表明，多乙烯磺酸钠在NK细胞裂解的结合后阶段抑制了一个与靶细胞相关的选择性过程。
