Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
Vitam Horm. 2014;94:427-35. doi: 10.1016/B978-0-12-800095-3.00015-8.
A recombinant Huh7-PPRE-Luc cell line, carrying a peroxisome proliferator response element (PPRE)-driven luciferase gene, is an efficient tool for evaluation of potential peroxisome proliferator-activated receptor (PPAR) agonists. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of PPAR agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Here, the bioassay is fitted into a 96-well plate format for high-throughput screening purpose.
一种携带过氧化物酶体增殖物激活受体（PPAR）反应元件（PPRE）驱动的荧光素酶基因的重组 Huh7-PPRE-Luc 细胞系是评估潜在过氧化物酶体增殖物激活受体（PPAR）激动剂的有效工具。该细胞系对三种亚型的 PPAR 激动剂以及视黄酸 X 受体激动剂 9-顺式视黄酸表现出良好的剂量反应诱导 PPRE 驱动的荧光素酶活性。在这里，该生物测定被适配到 96 孔板格式，用于高通量筛选目的。
