Institute of Cancer Research, School of Life Sciences and Technology, Xi'an Jiaotong University, Xi'an, P. R. China.
J Microbiol. 2014 Jan;52(1):77-82. doi: 10.1007/s12275-014-3235-9. Epub 2014 Jan 4.
Autotransporters have become attractive tools for surface expression of foreign proteins in Gram-negative bacteria. In this study, the Shigella autotransporter IcsA, has been exploited to express the human papillomavirus (HPV) type 16 L1 capsid protein in Shigella sonnei and Escherichia coli. The L1 gene was fused in-frame to replace the coding sequence of the IcsA passenger domain that is responsible for actin-based motility. The resultant hybrid protein could be detected by an anti-L1 antibody on the surface of S. sonnei and E. coli. In E. coli, the protein was expressed on the entire surface of the bacterium. In contrast, the protein was detected mainly at one pole of the Shigella bacterium. However, the protein became evenly distributed on the surface of the Shigella bacterium when the icsP gene was removed. Our study demonstrated the possibility of exploiting autotransporters for surface expression of large, heterologous viral proteins, which may be a useful strategy for vaccine development.
自转运蛋白已成为革兰氏阴性细菌中外源蛋白表面表达的有吸引力的工具。在这项研究中，志贺菌自转运蛋白 IcsA 被用于在志贺氏菌和大肠杆菌中表达人乳头瘤病毒（HPV）16 型 L1 衣壳蛋白。L1 基因与 IcsA 载体域的编码序列融合，该域负责基于肌动蛋白的运动。在 S. sonnei 和 E. coli 的表面可以用抗 L1 抗体检测到杂交蛋白。在大肠杆菌中，该蛋白在整个细菌表面表达。相比之下，该蛋白主要在志贺氏菌的一个极检测到。然而，当去除 icsP 基因时，该蛋白在志贺氏菌表面均匀分布。我们的研究表明，自转运蛋白可用于表面表达大型异源病毒蛋白，这可能是疫苗开发的有用策略。
