Bártek J, Bártková J, Schneider J, Taylor-Papadimitriou J, Kovarík J, Rejthar A
Eur J Cancer Clin Oncol. 1986 Dec;22(12):1441-52. doi: 10.1016/0277-5379(86)90077-5.
The monoclonal antibodies BA16 and BA17, reacting specifically with human keratin 19 (40 kD) have been tested by immunohistochemical staining methods for their reaction with a wide range of human tumours and cultured cells. Primary adenocarcinomas and their metastases showed a homogeneously positive reaction with greater than 95% of the tumour cells staining. Non-epithelial tumours, basaliomas and squamous cell carcinomas were unstained, while benign breast lesions and a thyroid adenoma show a mosaic pattern of stained and unstained (5-40%) cells. These three staining patterns were also seen in cultured cells. Positive homogeneous staining was seen in all breast cancer cell lines examined with the exception of PMC42, which exhibits stem cell characteristics, and which showed the heterogeneous pattern of staining seen in milk cell cultures. Non-epithelial lines and strains, two cell lines from cervical carcinomas and three SV40 transformed breast epithelial lines were unstained. The antibodies BA16 and 17 are potentially useful reagents for distinguishing adenocarcinomas (and their metastases) from non-epithelial tumours and from squamous carcinomas. They may also discriminate between benign and malignant breast lesions, and identify a specific differentiation phenotype in the secretory cell lineage.
单克隆抗体BA16和BA17能与人角蛋白19（40kD）特异性反应，已通过免疫组织化学染色方法检测它们与多种人类肿瘤及培养细胞的反应。原发性腺癌及其转移灶显示出均匀阳性反应，超过95%的肿瘤细胞染色。非上皮性肿瘤、基底细胞瘤和鳞状细胞癌未染色，而良性乳腺病变和甲状腺腺瘤显示出染色和未染色（5 - 40%）细胞的镶嵌模式。这三种染色模式在培养细胞中也可见。在所检测的所有乳腺癌细胞系中均可见阳性均匀染色，但具有干细胞特征的PMC42除外，它呈现出在乳腺细胞培养物中所见的异质性染色模式。非上皮细胞系和菌株、两个宫颈癌细胞系以及三个SV40转化的乳腺上皮细胞系未染色。抗体BA16和17是区分腺癌（及其转移灶）与非上皮性肿瘤和鳞状细胞癌的潜在有用试剂。它们还可区分良性和恶性乳腺病变，并识别分泌细胞谱系中的特定分化表型。
