Shires T K, Krieter P A, Shawver L K, Seidel S L
Fed Proc. 1987 Jun;46(8):2567-74.
The enzyme-linked immunosorbent assay (ELISA) has been investigated for its ability to quantitate hydrophobic proteins like cytochromes b5 and P-450 at the subnanogram level. Issues encountered that have broad significance not only for ELISA, but for other qualitative and quantitative immunoassays as well, include the effects of detergent, the discriminatory capacity of ELISA, and the method for determining an assay's selectivity.
酶联免疫吸附测定法（ELISA）已被研究用于定量检测亚纳克水平的疏水蛋白，如细胞色素b5和P - 450。所遇到的问题不仅对ELISA具有广泛意义，对其他定性和定量免疫测定法也具有广泛意义，包括去污剂的影响、ELISA的鉴别能力以及测定法选择性的确定方法。
